For in vitro use only!
Shipping: shipped on blue ice
Storage Conditions: store at -20 °C
Short term storage (up to 1 month) at 4 °C possible.
Shelf Life: 12 months
Form: liquid (Supplied in Jena Bioscience 6x Green Gel Loading Buffer (PCR-254-gr).
Concentration: 400 ng/μl
The Hinf I digest of pBR322 DNA yields the following 10 discrete fragments (in base pairs): 1632, 517, 504, 396, 344, 298, 221, 220, 154, 75.
Dilute in TE or other buffer of minimal ionic strength. DNA may denature if diluted in dH2O and subsequently heated.
2.5 μl per lane
pBR322 DNA (4.361 bp) was completely digested by Hinf I, phenol/chloroform extracted, ethanol precipitated, dissolved in 10 mM Tris-HCl (pH 8.0) and 1 mM EDTA.