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pBR322/ Hinf I Digest

ready-to-use

Product Cat. No. Amount Price (EUR) Buy / Note
pBR322/ Hinf I Digest M-107S 100 μg 48,00 Add to Basket/Quote Add to Notepad
pBR322/ Hinf I Digest M-107L 5 x 100 μg 192,00 Add to Basket/Quote Add to Notepad

1 μg loaded onto 1.7 % agarose

For in vitro use only!

Shipping: shipped on blue ice

Storage Conditions: store at -20 °C
Short term storage (up to 1 month) at 4 °C possible.

Shelf Life: 12 months

Form: liquid (Supplied in Jena Bioscience 6x Green Gel Loading Buffer (PCR-254-gr).

Concentration: 400 ng/μl

Description:
The Hinf I digest of pBR322 DNA yields the following 10 discrete fragments (in base pairs): 1632, 517, 504, 396, 344, 298, 221, 220, 154, 75.

Note:
Dilute in TE or other buffer of minimal ionic strength. DNA may denature if diluted in dH2O and subsequently heated.

Recommended Load:
2.5 μl per lane

Preparation:
pBR322 DNA (4.361 bp) was completely digested by Hinf I, phenol/chloroform extracted, ethanol precipitated, dissolved in 10 mM Tris-HCl (pH 8.0) and 1 mM EDTA.