-- λDNA/ Pst I Digest, Classic DNA Ladders
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λDNA/ Pst I Digest

ready-to-use

Product Cat. No. Amount Price (EUR) Buy / Note
λDNA/ Pst I Digest M-105S 100 μg 12,00 Add to Basket/Quote Add to Notepad
λDNA/ Pst I Digest M-105L 5 x 100 μg 48,00 Add to Basket/Quote Add to Notepad

1 μg loaded onto 1.5 % agarose

For in vitro use only!

Shipping: shipped on blue ice

Storage Conditions: store at -20 °C
Short term storage (up to 1 month) at 4 °C possible.

Shelf Life: 12 months

Form: liquid (Supplied in Jena Bioscience 6x Green Gel Loading Buffer (PCR-254-gr).

Concentration: 200 ng/μl

Description:
The Pst I digest of λ DNA yields the following discrete fragments (in base pairs): 11501*, 5077, 4749, 4507, 2838, 2556*, 2459, 2443, 2140, 1986, 1700, 1159 1093, 805, 514, 468, 448, 339, 264, 247, 216, 211, 200, 164, 150, 94, 87, 72, 15.

Note:
Dilute in TE or other buffer of minimal ionic strength. DNA may denature if diluted in dH2O and subsequently heated.

[*The cohesive ends (12b cos site of bacteriophage) of fragments 11501 bp and 2556 bp may anneal and form an additional band. These fragments may be separated again by heating to 65 °C for 5 min and then cooling on ice for 3 min.]

Recommended Load:
5 μl per lane

Preparation:
Lambda DNA (48.502 bp) was completely digested by Pst I, phenol extracted, ethanol precipitated, dissolved in 10 mM Tris-HCl (pH 8.0) and 1 mM EDTA.