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λDNA/ EcoR I/ Hind III Digest

ready-to-use

EcoRI

Product Cat. No. Amount Price (EUR) Buy / Note
λDNA/ EcoR I/ Hind III Digest M-103S 100 μg 12,00 Add to Basket/Quote Add to Notepad
λDNA/ EcoR I/ Hind III Digest M-103L 5 x 100 μg 48,00 Add to Basket/Quote Add to Notepad

1 μg loaded onto 1.0 % agarose

For in vitro use only!

Shipping: shipped on blue ice

Storage Conditions: store at -20 °C
Short term storage (up to 1 month) at 4 °C possible.

Shelf Life: 12 months

Form: liquid (Supplied in Jena Bioscience 6x Green Gel Loading Buffer (PCR-254-gr).

Concentration: 200 ng/μl

Description:
The EcoR I and Hind III digest of λ DNA yields the following 13 discrete fragments (in base pairs): 21226*, 5148, 4973, 4268, 3530*, 2027, 1904, 1584, 1375, 947, 831, 564, 125.

Note:
Dilute in TE or other buffer of minimal ionic strength. DNA may denature if diluted in dH2O and subsequently heated.

[*The cohesive ends (12b cos site of bacteriophage lambda) of fragments 21226 bp and 3530 bp may anneal and form an additional band. These fragments may be separated again by heating to 65 °C for 5 min and then cooling on ice for 3 min.]

Recommended Load:
5 μl per lane

Preparation:
Lambda DNA (48.502 bp) was completely digested by EcoR I and Hind III, phenol extracted, ethanol precipitated, dissolved in 10 mM Tris-HCl (pH 8.0) and 1 mM EDTA.