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λDNA/ EcoR I Digest

ready-to-use

Product Cat. No. Amount Price (EUR) Buy / Note
λDNA/ EcoR I Digest M-102S 50 μg 12,00 Add to Basket/Quote Add to Notepad
λDNA/ EcoR I Digest M-102L 5 x 50 μg 48,00 Add to Basket/Quote Add to Notepad

0.1 μg loaded onto 0.7 % agarose

For in vitro use only!

Shipping: shipped on blue ice

Storage Conditions: store at -20 °C
Short term storage (up to 1 month) at 4 °C possible.

Shelf Life: 12 months

Form: liquid (Supplied in Jena Bioscience 6x Green Gel Loading Buffer (PCR-254-gr).

Concentration: 50 ng/μl

Description:
The EcoR I digest of λ DNA yields the following 6 discrete fragments (in base pairs): 21226*, 7421, 5804, 5643, 4878, 3530*.

Note:
Dilute in TE or other buffer of minimal ionic strength. DNA may denature if diluted in dH2O and subsequently heated.

[*The cohesive ends (12b cos site of bacteriophage lambda) of fragments 21226 bp and 3530 bp may anneal and form an additional band. These fragments may be separated again by heating to 65 °C for 5 min and then cooling on ice for 3 min.]

Recommended Load:
2 - 5 μl per lane

Preparation:
Lambda DNA (48.502 bp) was completely digested by EcoR I, phenol extracted, ethanol precipitated, dissolved in 10 mM Tris-HCl (pH 8.0) and 1 mM EDTA.