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λDNA/ Hind III Digest

ready-to-use

Product Cat. No. Amount Price (EUR) Buy / Note
λDNA/ Hind III Digest M-101S 100 μg 12,00 Add to Basket/Quote Add to Notepad
λDNA/ Hind III Digest M-101L 5 x 100 μg 48,00 Add to Basket/Quote Add to Notepad

1 μg loaded onto 1.0 % agarose

For in vitro use only!

Shipping: shipped on blue ice

Storage Conditions: store at -20 °C
Short term storage (up to 1 month) at 4 °C possible.

Shelf Life: 12 months

Form: liquid (Supplied in Jena Bioscience 6x Green Gel Loading Buffer (PCR-254-gr).

Concentration: 200 ng/μl

Description:
The Hind III digest of λ DNA yields the following 8 discrete fragments (in base pairs): 23130*, 9416, 6557, 4361*, 2322, 2027, 564, 125.

Note:
Dilute in TE or other buffer of minimal ionic strength. DNA may denature if diluted in dH2O and subsequently heated.

[*The cohesive ends (12b cos site of bacteriophage lambda) of fragments 23130 bp and 4361 bp may be separated by heating to
65 °C for 5 min.]

Recommended Load:
5 μl per lane

Preparation:
Lambda DNA (48.502 bp) was completely digested by Hind III, phenol extracted, ethanol precipitated, dissolved in 10 mM Tris-HCl (pH 8.0) and 1 mM EDTA.