For in vitro use only!
Shipping: shipped on blue ice
Storage Conditions: store at -20 °C
Short term storage (up to 1 month) at 4 °C possible.
Shelf Life: 12 months
Form: liquid (Supplied in Jena Bioscience 6x Green Gel Loading Buffer (PCR-254-gr).
Concentration: 200 ng/μl
The Hind III digest of λ DNA yields the following 8 discrete fragments (in base pairs): 23130*, 9416, 6557, 4361*, 2322, 2027, 564, 125.
* The cohesive ends (12b cos site of bacteriophage lambda) of fragments 23130 bp and 4361 bp may be separated by heating to 65 °C for 5 min.
Dilute in TE or other buffer of minimal ionic strength. DNA may denature if diluted in dH2O and subsequently heated.
5 μl per lane
Lambda DNA (48.502 bp) was completely digested by Hind III, phenol extracted, ethanol precipitated, dissolved in 10 mM Tris-HCl (pH 8.0) and 1 mM EDTA.