The CyClone Ligase-free Cloning Kit generates plasmids usable for transformation in only two simple steps:
1) Creation of a PCR product with ends homologous to the cloning vector.
2) Annealing and fusion of insert and vector in a circularization reaction.
No time consuming ligation step is necessary!
A specialized software, allowing convenient primer design and ordering for the use of CyClone with our LEXSY vectors, is located here (web shop account required).