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PI3K Lipid Substrate Mix 2

PI-4,5-P2, PE, PS, PC, and SM

based on PI-4,5-P2

Cat. No. Amount Price (EUR) Buy / Note
LI-012 100 μg 190,00 Add to Basket/Quote Add to Notepad

For in vitro use only!

Shipping: shipped on blue ice

Storage Conditions: store at -20 °C

Shelf Life: 12 months

Molecular Formula: C41H81NO19P3

Molecular Weight: 970.99 g/mol

Purity: ≥ 98 %

Form: lyophilized

Solubility: soluble in Chloroform, yields a suspension in aqueous solution

Description:
The PI3K Lipid Substrate Mix 2 is recommended for PI3K kinase activity assays (Maier et al., 1999; Stephens et al. 1993). Phosphoinositide 3-kinases (PI3Ks) phosphorylate phosphatidylinositols (PIs) at their 3’ OH position generating lipid second messengers and thereby regulate numerous biological processes including cell growth, differentiation, survival, proliferation, migration and metabolism.

Applications:
Dissolve the mix in 200 μl Chloroform and take an aliquot of 3 μl per reaction. Evaporate the chloroform (for fast evaporation use a stream of nitrogen) and resuspend in 30 μl kinase buffer. Sonicate for 1 h in a water bath. The total kinase assay reaction volume should be 50 μl.

Composition:
4.92% L-α-Phosphatidylinositol-4,5-bisphosphate
(PI-4,5-P2)
36.07% L-α-Phosphatidylethanolamine (PE)
39.34% L-α-Phosphatidyl-L-serine (PS)
16.39% L-α-Lysophosphatidylcholine (PC)
3.28% Sphingomyelin (SM).

Selected References:
Maier et al. (1999) Roles of Non-catalytic Subunits in Gβγ-induced Activation of Class I Phosphoinositide 3-Kinase Isoforms β and γ. J. Biol. Chem. 274:29311.
Stephens et al. (1993) Synthesis of phosphatidylinositol 3,4,5-trisphosphate in permeabilized neutrophils regulated by receptors and G-proteins. J. Biol. Chem. 268:17162.
Vanhaesebroeck et al. (2001) Synthesis and function of 3-phosphorylated inositol lipids. Ann. Rev. Biochem. 70:535.
Balla (2001) Pharmacology of phosphoinositides, regulators of multiple cellular functions. Curr. Pharm. Des. 7:475.
Wymann (2003) Phosphoinositide 3-kinase signalling – which way to target? Trend Pharmacol. Sci. 24:323.
Foukas et al. (2002) Direct effects of caffeine and theophylline on p110 delta and other phosphoinositide 3-kinases. Differential effects on lipid kinase and protein kinase activities. J. Biol. Chem. 277:37124.