PI-4,5-P2, PE, PS, PC, and SM
based on PI-4,5-P2
For in vitro use only!
Shipping: shipped on blue ice
Storage Conditions: store at -20 °C
Shelf Life: 12 months
Molecular Formula: C41H81NO19P3
Molecular Weight: 970.99 g/mol
Purity: ≥ 98 %
Solubility: soluble in Chloroform, yields a suspension in aqueous solution
The PI3K Lipid Substrate Mix 2 is recommended for PI3K kinase activity assays (Maier et al., 1999; Stephens et al. 1993). Phosphoinositide 3-kinases (PI3Ks) phosphorylate phosphatidylinositols (PIs) at their 3’ OH position generating lipid second messengers and thereby regulate numerous biological processes including cell growth, differentiation, survival, proliferation, migration and metabolism.
Dissolve the mix in 200 μl Chloroform and take an aliquot of 3 μl per reaction. Evaporate the chloroform (for fast evaporation use a stream of nitrogen) and resuspend in 30 μl kinase buffer. Sonicate for 1 h in a water bath. The total kinase assay reaction volume should be 50 μl.
36.07% L-α-Phosphatidylethanolamine (PE)
39.34% L-α-Phosphatidyl-L-serine (PS)
16.39% L-α-Lysophosphatidylcholine (PC)
3.28% Sphingomyelin (SM).
Maier et al. (1999) Roles of Non-catalytic Subunits in Gβγ-induced Activation of Class I Phosphoinositide 3-Kinase Isoforms β and γ. J. Biol. Chem. 274:29311.
Stephens et al. (1993) Synthesis of phosphatidylinositol 3,4,5-trisphosphate in permeabilized neutrophils regulated by receptors and G-proteins. J. Biol. Chem. 268:17162.
Vanhaesebroeck et al. (2001) Synthesis and function of 3-phosphorylated inositol lipids. Ann. Rev. Biochem. 70:535.
Balla (2001) Pharmacology of phosphoinositides, regulators of multiple cellular functions. Curr. Pharm. Des. 7:475.
Wymann (2003) Phosphoinositide 3-kinase signalling which way to target? Trend Pharmacol. Sci. 24:323.
Foukas et al. (2002) Direct effects of caffeine and theophylline on p110 delta and other phosphoinositide 3-kinases. Differential effects on lipid kinase and protein kinase activities. J. Biol. Chem. 277:37124.