for inducible expression
For research use only! Not intended for human or animal diagnostic or therapeutic uses.
Shipping: shipped on dry ice
Storage Conditions: store at -80 °C
avoid freeze/thaw cycles
Upon arrival the glycerol stocks must be stored at -80°C or inoculated into LEXSY BHI.
Shelf Life: 12 months
The Leishmania tarentolae strain T7-TR is used as host strain for the inducible LEXSY expression vectors. Please, refer to corporate website for vector details.
The product contains:
3 vials with 1.6 ml each of frozen glycerol stocks of LEXSY host T7-TR (recombinant Leishmania tarentolae strain expressing bacteriophage T7 RNA polymerase and TET repressor).
These stocks can be stored at -80°C for at least 1 year. For reactivation see below.
Recombinant Leishmania tarentolae, strain constitutively expressing bacteriophage T7 RNA polymerase and TET repressor.
1, Non-pathogenic for mammalians
Reactivation of LEXSY host:
Thaw glycerol stock on ice and inoculate the entire content of the vial into 10 ml of LEXSY BHI medium (Cat.-No. ML-411) containing the antibiotics Nourseothricin (NTC) (Cat.-No. AB-101) and LEXSY Hygro. Incubate at 26°C and dilute as required.
Preparation of LEXSY BHI growth medium:
Dissolve 37 g/l LEXSY BHI powder (Cat.-No. ML-412) in deionized water and autoclave 15 min at 121°C. Add Hemin and PenStrep. Store at 4°C and use within two weeks.
Immediately before use, add the antibiotics Nourseothricin (NTC) (Cat.-No. AB-101) and LEXSY Hygro. (Cat.-No. AB-104) for maintenance of the T7 polymerase and TET repressor genes.
Preparation of glycerol stocks:
Add 1.2 ml of growing culture (ca. 6x107 cells/ml) to one vial with 0.4 ml 80% glycerol, mix, incubate 10 min at RT, 1 h on ice and o/n at -20°C. Transfer to -80°C. Strains can be stored this way for several years.