LEXSY host P10 - glycerol stocks
For research use only. Not intended for human or animal diagnostic or therapeutic uses.
Description:
The Leishmania tarentolae laboratory strain P10 is used as host strain for the constitutive LEXSY expression vectors.
Shipping: Shipped on dry ice
Storage Conditions: Store at -80 °C
Avoid freeze/thaw cycles
Upon arrival the glycerol stocks must be stored at -80 °C or inoculated into LEXSY BHI.
Shelf Life: 12 months
Content:
3 vials with 1.6 ml each of frozen glycerol stocks of LEXSY host P10 (Leishmania tarentolae laboratory strain P10).
These stocks can be stored at -80 °C for at least 1 year. For reactivation see below.
Organism:
Leishmania tarentolae, laboratory strain P10
Biosafety level:
1, Non-pathogenic for mammalians
Source:
Tarentola annularis
Reactivation of LEXSY host:
Thaw glycerol stock on ice and inoculate the entire content of the vial into 10 ml of LEXSY BHI medium (Cat.-No. ML-411). Incubate at 26 °C and dilute as required.
Preparation of LEXSY BHI growth medium:
Dissolve 37 g/l LEXSY BHI powder (Cat.-No. ML-412) in deionized water and autoclave for 15 min at 121 °C. Add Hemin and PenStrep. Store at 4 °C and use within two weeks.
Preparation of glycerol stocks:
Add 1.2 ml of growing culture (ca. 6x107 cells/ml) to one vial with 0.4 ml 80 % glycerol, mix, incubate 10 min at RT, 1 h on ice and over night at -20 °C. Transfer to -80 °C. Strains can be stored this way for several years.
Selected References:
[1] Zauner et al. (2018) Structural Analyses of Arabidopsis thaliana Legumain γ Reveal Differential Recognition and Processing of Proteolysis and Ligation Substrates. J Biol Chem 293: 8934
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Scientific citations for Jena Bioscience lt-101
29 scientific citations and 11 citation images found by Bioz.
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Exploring elastin-like polypeptide tags and mini-intein for recombinant protein purification in Leishmaniatarentolae.
Protein expression and purification, 2026-07-08 · PMID:41722743
Abstract: Recombinant technology has enabled the production of a wide range of proteins with applications in various fields, including reproduction, food supplements, and medicine. However, the high costs associated with producing and purifying recombinant proteins limit their widespread use in the veterinary field. Therefore, the development of alternative, low-cost strategies for production and purification is essential to address these challenges
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Axonemal dynein contributions to flagellar beat types and waveforms
bioRxiv, 2025-11-07 · DOI:10.1101/2025.11.06.687033
PrePrint: Leishmania tarentolae strain P10 (Jena Bioscience, #
LT-101 -
Identification of small molecule inhibitors of Trypanosoma PEX15– PEX6 interaction
bioRxiv, 2025-10-04 · DOI:10.1101/2025.10.03.680009
PrePrint: .. logarithmic phase (below 1-2 × 10 6 cells/ml as described in [ , ]).. Leishmania tarentolae promastigotes (
LEXSY host P10 ,Jena Bioscience ) were grown in LEXSY Broth BHI medium (Jena Biosciences) supplemented with 7.5 μg/mL hemin.. HepG2 (hepatocyte) cells were used to study the cytotoxicity of compounds.HepG2 (hepatocyte) cells were ..
