Recombinant production of antibodies with focus on monoclonal antibodies (MAbs) has become a challenging task due to the rapidly expanding pharmaceutical and diagnostic markets.
LEXSY was evaluated for production of heavy and light chains of human IgG, single chain antibodies and Fc fusions. Recombinant Fc fusions were efficiently expressed in LEXSY, completely secreted to the culture medium and one-step affinity purified with Protein A sepharose with yields of ca. 10 mg/L. SDS PAGE analysis demonstrated that the proteins were secreted in the native configuration as dimers (JBS not published).
Purification of Fc fusion protein from LEXSY cultivation medium. Lane 1 molecular size marker, 2-3 Protein A sepharose-purified Fc fusion under reducing conditions, 4 dto. non-reducing conditions (JBS not published).