PI 3-Kinase Inhibitor
For in vitro use only!
Shipping: shipped on blue ice
Storage Conditions: store at -20 °C
Shelf Life: 12 months
Molecular Formula: C23H24O8
Molecular Weight: 428.4 g/mol
Purity: ≥ 98 %
Solubility: 25 mg/ml soluble in DMSO, 5 mg/ml in Ethanol. Insoluble in water.
Wortmannin is a cell-permeable, fungal metabolite that acts as a potent, selective and irreversible inhibitor of phosphatidylinositol 3-kinase (PI3K). It is active with purified preparations, cytosolic fractions and suitable for use with intact cells.
Wortmannin blocks the catalytic activity of PI3-Kinase with an IC50 of 5 nM and without affection of upstream signalling events.
It also inhibits the activities of myosin light chain kinase (MLCK), PI4-Kinase and phospholipase C (PLC) but at concentrations about 100 fold higher than those required for PI3K inhibition.
Arcaro et al. (1993) Wortmannin is a potent phosphatidylinositol 3-kinase inhibitor: the role of phosphatidylinositol 3,4,5-trisphosphate in neutrophil responses. Biochem. J. 296:297.
Wymann et al. (1994) Platelet-derived growth factorinduced phosphatidylinositol 3-kinase activation mediates actin rearrangements in fibroblasts. Biochem J. 298:517.
Walker et al. (2000) Structural Determinants of Phosphoinositide 3-Kinase Inhibition by Wortmannin, LY294002, Quercetin, Myricetin,and Staurosporine. Mol. Cell. 6:909.
Houle et al. (2003) Wortmannin alters the intracellular trafficking of the bradykinin B2 receptor: role of phosphoinositide 3-kinase and Rab5. Biochem. J. 375:151.