0 Water 1 0 Water 1 0 Water 1 0 Water 1 0 Water 1 0 Water 1 0 Water 1 0 Water 1 0 Water 1 0 Water 1 0 Water 1 0 Water 1 0.5 3 Buffer 2 0.5 3.8 Buffer 7 8 0.5 3.2 Buffer 11 12 0.5 3.5 Buffer 13 14 0.5 4.5 Buffer 16 17 0.5 4.5 Buffer 20 21 0.5 8.5 Buffer 25 26 0.5 8.5 Buffer 27 28 0.5 8.5 Buffer 29 30 0.5 10 Buffer 3 4 0.5 9.5 Buffer 31 32 0.5 9 Buffer 22 23 0.5 3 Buffer 34 35 0.5 3 Buffer 36 37 0.5 3.5 Buffer 11 12 0.5 4 Buffer 13 14 0.5 5 Buffer 16 17 0.5 5 Buffer 20 21 0.5 9 Buffer 25 26 0.5 9 Buffer 27 28 0.5 9 Buffer 29 30 0.5 10.5 Buffer 3 4 0.5 10 Buffer 31 32 0.5 9.5 Buffer 22 23 0.5 3.5 Buffer 34 35 0.5 3.5 Buffer 36 37 0.5 4 Buffer 11 12 0.5 4.5 Buffer 13 14 0.5 5.5 Buffer 16 17 0.5 5.5 Buffer 20 21 0.5 9.5 Buffer 25 26 0.5 8.5 Buffer 38 39 0.5 9.5 Buffer 29 30 0.5 8.6 Buffer 40 41 0.5 9.7 Buffer 42 43 0.5 10 Buffer 22 23 0.5 4 Buffer 34 35 0.5 4 Buffer 36 37 0.5 4.5 Buffer 11 12 0.5 5 Buffer 13 14 0.5 5 Buffer 44 45 0.5 4 Buffer 46 47 0.5 8.5 Buffer 9 10 0.5 9 Buffer 38 39 0.5 8.5 Buffer 50 0.5 9 Buffer 40 41 0.5 10 Buffer 42 43 0.5 8.5 Buffer 48 49 0.5 4.5 Buffer 34 35 0.5 4.5 Buffer 36 37 0.5 5 Buffer 11 12 0.5 5.5 Buffer 13 14 0.5 5.5 Buffer 44 45 0.5 4.5 Buffer 46 47 0.5 8.9 Buffer 9 10 0.5 9.5 Buffer 38 39 0.5 8.6 Buffer 5 6 0.5 9.5 Buffer 40 41 0.5 10.5 Buffer 42 43 0.5 9 Buffer 48 49 0.5 3 Buffer 7 8 0.5 5 Buffer 36 37 0.5 5.5 Buffer 11 12 0.5 3.6 Buffer 18 19 0.5 5.5 Buffer 51 0.5 5 Buffer 46 47 0.5 8.5 Buffer 52 53 0.5 8.5 Buffer 54 55 0.5 9 Buffer 5 6 0.5 10 Buffer 40 41 0.5 11 Buffer 42 43 0.5 9.5 Buffer 48 49 0.5 3.5 Buffer 7 8 0.5 5.5 Buffer 36 37 0.5 3.2 Buffer 13 15 0.5 4 Buffer 18 19 0.5 4 Buffer 20 0.5 5.5 Buffer 46 47 0.5 9 Buffer 52 53 0.5 9 Buffer 54 55 0.5 9.5 Buffer 5 6 0.5 9 Buffer 33 0.5 8.5 Buffer 24 22 0.5 10 Buffer 48 49 1 100 0 0 % false Water Water Water 2 1 0.1 0.1 M 3 true Jena Bioscience 3 1 0.1 0.1 M 10 true 50046 Sigma 4 1 0.1 0.1 M 10.5 true Qiagen 5 1 0 1 M 8 true Jena Bioscience 6 1 0.1 0.1 M 9.5 true Hampton Research Aminoacetic acid Aminoethanoic acid Glycocoll Buffer 9.8 56-40-6 Glycine Gly 7 1 0 1 M 3 true 8 1 0 1 M 4 true 9 1 0.1 0.1 M 8.5 true Fluidigm 10 1 0 1 M 9 true diglycine Buffer 8.3 556-50-3 Glycyl-glycine Gly2 11 1 0.1 0.1 M 3.2 true Jena Bioscience Citric acid, pH has been adjusted to 3.2 using NaOH 12 1 0.1 0.1 M 5.5 true Jena Bioscience tri-Sodium citrate titrated with citric acid Citric acid trisodium salt dihydrate Trisodium citrate dihydrate Sodium citrate tribasic dihydrate Buffer: Citric Acid Buffer 7.5 0.001 3 2.42 3.1 2.36 3.2 2.29 3.3 2.22 3.4 2.15 3.5 2.08 3.6 2.01 3.7 1.93 3.8 1.85 3.9 1.78 4 1.7 4.1 1.62 4.2 1.54 4.3 1.46 4.4 1.38 4.5 1.3 4.6 1.22 4.7 1.15 4.8 1.07 4.9 0.99 5 0.92 5.1 0.85 5.2 0.77 5.3 0.71 5.4 0.64 5.5 0.57 5.6 0.51 5.7 0.45 5.8 0.4 5.9 0.34 6 0.29 6.1 0.25 6.2 0.2 6.3 0.16 6.4 0.12 6.5 0.08 2 2.9 1 10 6132-04-3 68-04-2 77-92-9 tri-Sodium citrate Na3 Cit 13 1 0 1 M 3 true 14 1 0 0.1 M 5.5 true CP-Buffer-13 Axygen 15 1 0 0.1 M 4.5 true CP-Buffer-12 Axygen dicarboxylic acid C4 butanedioic acid Succinic Acid Buffer 5.6 110-15-6 150-90-3 di-Sodium succinate Na2 Succ 16 1 0.1 0.1 M 4.5 true CP-Buffer-31 Axygen 17 1 0.1 0.1 M 5.5 true Emerald Titrated to pH 5.5 using HCl 18 1 0 1 M 3.4 true Emerald 19 1 0.1 0.1 M 4 true HR2-933-05 Hampton Research Titrated to pH 4.0 using HCl Acetic acid sodium salt Sodium acetate trihydrate Buffer 4.8 6131-90-4 64-19-7 127-09-3 Sodium acetate Na Acet 20 1 0.1 0.1 M 4 true Jena Bioscience MIB buffer is produced by mixing sodium malonate, imidazole, and boric acid in the molar ratios 2:3:3 – sodium malonate:imidazole:boric acid.* The three chemicals have three different buffering curves and their ratios have been selected to keep pH variation almost linear. The desired pH is obtained by mixing high- and low-pH stock solutions 21 1 0.1 0.1 M 6 true Jena Bioscience MIB buffer is produced by mixing sodium malonate, imidazole, and boric acid in the molar ratios 2:3:3 – sodium malonate:imidazole:boric acid.* The three chemicals have three different buffering curves and their ratios have been selected to keep pH variation almost linear. The desired pH is obtained by mixing high- and low-pH stock solutions 22 1 0.1 0.1 M 9 true Jena Bioscience MIB buffer is produced by mixing sodium malonate, imidazole, and boric acid in the molar ratios 2:3:3 – sodium malonate:imidazole:boric acid.* The three chemicals have three different buffering curves and their ratios have been selected to keep pH variation almost linear. The desired pH is obtained by mixing high- and low-pH stock solutions 23 1 0.1 0.1 M 10 true Molecular Dimensions MIB buffer is produced by mixing sodium malonate, imidazole, and boric acid in the molar ratios 2:3:3 – sodium malonate:imidazole:boric acid.* The three chemicals have three different buffering curves and their ratios have been selected to keep pH variation almost linear. The desired pH is obtained by mixing high- and low-pH stock solutions 24 1 0.1 0.1 M 8 true Jena Bioscience MIB buffer is produced by mixing sodium malonate, imidazole, and boric acid in the molar ratios 2:3:3 – sodium malonate:imidazole:boric acid.* The three chemicals have three different buffering curves and their ratios have been selected to keep pH variation almost linear. The desired pH is obtained by mixing high- and low-pH stock solutions Buffer 4 10 10 0.02 141-82-2 288-32-4 10043-35-3 MIB buffer MIB 25 1 0.1 0.1 M 8.5 true Jena Bioscience 26 1 0.1 0.1 M 9.7 true HR2-833 Hampton Research 1,3-Bis[tris(hydroxymethyl)methylamino]propane Buffer 6.8 64431-96-5 BIS-TRIS propane BIS-TRIS prop 27 1 0.1 0.1 M 8.5 true HR2-999-10 Hampton Research pH has been adjusted to 8.5 using NaOH 28 1 0.1 0.1 M 9 true Jena Bioscience N,N-Bis(2-hydroxyethyl)glycine Buffer 8.3 150-25-4 BICINE BICINE 29 1 0 1 M 8.5 true 30 1 0 1 M 9.5 true Buffer 7 107-35-7 2-Aminoethanesulfonic acid Taurine 31 1 0.1 0.1 M 9.5 true Jena Bioscience 32 1 0 1 M 10 true 33 1 0.1 0.1 M 9 true Jena Bioscience 3-(Cyclohexylamino)-2-hydroxy-1-propanesulfonic ac Buffer 9.6 CAPSO CAPSO 34 1 0 1 M 3 true 35 1 0 1 M 4.5 true Formic acid sodium salt Buffer 3.7 141-53-7 Sodium formate Na Form 36 3 0 3 M 2.9 true Microlytic 37 3 0.1 0.1 M 7 true Jena Bioscience DL Malic acid, titrated with NaOH (±)-2-Hydroxysuccinic acid DL-Hydroxybutanedioic acid DL-Malic Acid Buffer 2.19 10.01133567 2.23 9.101214246 2.27 8.342779725 2.31 7.701027439 2.34 7.15095405 2.37 6.67422378 2.4 6.257084794 2.46 5.56185315 2.51 5.005667835 2.56 4.550607123 2.61 4.171389863 2.66 3.850513719 2.7 3.575477025 2.74 3.33711189 2.79 3.080410975 2.84 2.86038162 2.88 2.669689512 2.92 2.502833918 2.97 2.355608393 3.01 2.22474126 3.05 2.107649615 3.09 2.002267134 3.13 1.90692108 3.17 1.820242849 3.2 1.741101856 3.24 1.668555945 3.27 1.601813707 3.31 1.540205488 3.35 1.48316084 3.39 1.43019081 3.42 1.380873886 3.46 1.334844756 3.5 1.291785248 3.53 1.251416959 3.59 1.191825675 3.65 1.137651781 3.71 1.08818866 3.76 1.042847466 3.82 1.001133567 3.87 0.96262843 3.93 0.926975525 3.99 0.893869256 4.05 0.863046178 4.11 0.834277973 4.15 0.817251891 4.18 0.800906854 4.22 0.785202798 4.26 0.770102744 4.29 0.755572503 4.32 0.74158042 4.36 0.72809714 4.4 0.715095405 4.44 0.702549872 4.48 0.690436943 4.51 0.678734622 4.55 0.667422378 4.58 0.656481028 4.62 0.645892624 4.67 0.63564036 4.71 0.625708479 4.75 0.616082195 4.79 0.606747616 4.84 0.597691682 4.89 0.588902098 4.94 0.580367285 4.99 0.572076324 5.05 0.564018911 5.12 0.556185315 5.17 0.550073388 5.22 0.54409433 5.29 0.538243853 5.36 0.532517855 5.4 0.5297003 5.45 0.526912404 5.6 0.518722055 5.5 0.5241537 5.55 0.521423733 5.67 0.51604823 5.75 0.513401829 5.79 0.512088781 5.82 0.510782432 5.88 0.509482731 5.95 0.508189628 6.03 0.506903072 6.08 0.505623014 6.16 0.504349404 6.25 0.503082194 6.4 0.501821337 6.6 0.500566784 6.98 0.499318487 10.16 0.498076401 6915-15-7 di-Sodium DL-malate Na2 Mal (DL) 38 1 0 1 M 8 true 39 1 0.1 0.1 M 10 true Molecular Dimensions 2-Amino-2-methyl-1,3-propanediol Buffer 8.8 115-69-5 AMPD AMPD 40 1 0 1 M 8 true Emerald 41 1 0.1 0.1 M 10 true 133174 Qiagen 2-(Cyclohexylamino)ethanesulfonic acid Buffer 9.5 103-47-9 CHES CHES 42 1 0.1 0.1 M 9.5 true Emerald 43 1 0.1 0.1 M 11.5 true Emerald 3-(Cyclohexylamino)-1-propanesulfonic acid Buffer 10.5 1135-40-6 CAPS CAPS 44 3.4 0.1 0.1 M 5 true 133243 Qiagen 45 3.4 0.1 1.4 M 6 true Molecular Dimensions Propanedioic acid Buffer 5.5 141-82-2 141-95-7 di-Sodium malonate Na2 Malon 46 1 0.1 0.1 M 4 true Jena Bioscience 47 1 0.1 0.1 M 5.5 true Jena Bioscience 48 1 0.1 0.1 M 8.5 true Jena Bioscience 49 1 0.1 0.1 M 10 true 133277 Qiagen Buffer 4 10 10 0.02 110-15-6 7558-80-7 56-40-6 SPG buffer SPG 50 1 0.1 0.1 M 8.5 true 133281 Qiagen N-(tris(hydroxymethyl)methyl)glycine Buffer 8.1 TRICINE TRICINE 51 1 0.1 0.1 M 5.5 true CP-Buffer-20 Axygen 2-(N-morpholino)ethanesulfonic acid 4-morpholineethanesulfonic acid monohydrate MES monohydrate 2-Morpholinoethanesulfonic acid Buffer 6.1 145224-94-8 4432-31-9 71119-23-8 MES MES 52 1 0.1 0.1 M 8.5 true Jena Bioscience 53 1 0.1 0.1 M 9 true Axygen Trizma base THAM Tris(hydroxymethyl)aminomethane Trometamol Tris hydrochloride 2-Amino-2-(hydroxymethyl)-1,3-propanediol Buffer 8.1 77-86-1 1185-53-1 TRIS TRIS 54 1 0.1 0.1 M 8.5 true Jena Bioscience 55 1 0.1 0.1 M 9 true Fluidigm N-[Tris(hydroxymethyl)methyl]-3-amino propanesul Buffer 8.4 29915-38-6 TAPS TAPS