Logo Jena Bioscience - Building Blocks of Life

XP Screen: The Protein Crystal Glue

XP Screen: The protein crystal glue

Figure adapted from [1], used by courtesy of Prof. Annette Rompel, University of Vienna, Austria

    The Anderson−Evans polyoxotungstate [TeW6O24]6− (TEW) is a universal and flexible additive for protein crystallization. With its planar structure and a central tellurium atom, it is able to stabilize flexible regions in proteins and provides an anomalous signal for phasing[1].

    TEW was shown to:

    • covalently bind and structurally adapt to fit into protein molecules (cgAUS1, PDB code: 4Z12, 4Z13)[2]
    • act as linker in various orientations and thereby create either smaller (abPPO4, PDB code: 4OUA) or larger (lysozyme, PDB code: 4PHI) protein-protein distances[3,4]
    • induce heterogeneous crystallization of two different protein forms in one single crystal (abPPO4, PDB code: 4OUA)[4]

    Our new XP Screen contains TEW in 96 of the most prominent crystallization conditions, providing an environment for TEW to improve crystallization and crystal diffraction quality especially for challenging targets.

    [1] Bijelic et al. (2017) Ten Good Reasons for the Use of the Tellurium-Centered Anderson-Evans Polyoxotungstate in Protein Crystallography. Acc. Chem. Res. 50:1441.
    [2] Molitor et al. (2016) In situ formation of the first proteinogenically functionalized [TeW6O24O2(Glu)]7- structure reveals unprecedented chemical and geometrical features of the Anderson-type cluster. Chem. Commun. 52:12286.
    [3] Bijelic et al. (2015) Hen Egg-White Lysozyme Crystallisation: Protein Stacking and Structure Stability Enhanced by a Tellurium(VI)-Centred Polyoxotungstate. ChemBioChem 16:233.
    [4] Mauracher et al. (2014) Latent and active abPPO4 mushroom tyrosinase cocrystallized with hexatungstotellurate(VI) in a single crystal. Acta Cryst. D 70:2301.

    Responsible for content / Imprint:
    Jena Bioscience GmbH
    Loebstedter Str. 71 | 07749 Jena | Germany
    Phone: +49 – 3641 – 6285 000 | Fax: +49 – 3641 – 6285 100 | E-Mail: info@jenabioscience.com

    Register Court:
    Amtsgericht Jena, HRB 207171 | VAT No.: DE 195825742

    Managing Directors: Thomas Billert | Dr. Mathias Grün

    Jena Bioscience Facebook Jena Bioscience Google+ Jena Bioscience Youtube Jena Bioscience LinkedIn