QUANTIFOIL™ Holey Carbon Films

Supporting grids for Cryo-EM

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Cryo-electron microscopy (Cryo-EM) is a technique that uses accelerated electrons as illumination source for biological samples.

To minimize radiation damage, the sample is kept at cryogenic temperatures and the electron dose has to be low – resulting in noisy images. However, the enormous progress in hardware development and image processing over the past few years has made the technique available to solve near-atomic-resolution protein structures.

Cryo-EM image of the Chaperone GroEL suspended in amorphous ice at 50.000x magnification.
Image source: Wikipedia (modified)

QUANTIFOIL™ Holey Carbon Films provide ideal support for biological samples in Cryo-EM techniques, such as electron crystallography, single particle analysis and MicroED[1-6]:
  • 12 nm carbon film with circular holes of defined size and interspace for various magnifications
  • The circular holes result in formation of an ice layer with constant thickness
  • Supporting mesh: copper or gold

[1] Park et al. (2017) Structure of a CLC chloride ion channel by cryo-electron microscopy. Nature 541:500.
[2] Kalynych et al. (2017) Cryo-EM study of slow bee paralysis virus at low pH reveals iflavirus genome release mechanism. PNAS 114:598.
[3] Ekiert et al. (2017) Architectures of Lipid Transport Systems for the Bacterial Outer Membrane. Cell 169:273.
[4] Beniac et al. (2017) Structure of the Ebola virus glycoprotein spike within the virion envelope at 11 Å resolution. Sci. Rep. DOI: 10.1038/srep46374.
[5] Lokareddy et al. (2017) Portal protein functions akin to a DNA-sensor that couples genome-packaging to icosahedral capsid maturation. Nat. Commun. DOI: 10.1038/ncomms14310.
[6] Davies et al. (2017) Cryo-EM analysis of a domain antibody bound rotary ATPase complex. J. Struct. Biol. 197:350.

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