LEXSY for difficult-to-express Proteins

(1) LEXSY is successful where other expression systems fail

(2) LEXSY – everything for cloning, expression strain selection, and cultivation


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   (1) LEXSY is successful where other expression systems fail

LEXSY (Leishmania Expression System) is a eukaryotic protein expression platform based on the S1 classified protozoan L. tarentolae.

It combines the advantages of prokaryotic systems (easy handling, fast growth) with those of eukaryotic ones (efficient folding, posttranslational modifications).

Figure 1: LEXSY cells expressing green fluorescent protein.

Of more than 70 proteins, whose expression in other systems like E. coli, yeast, insect or mammalian cells was unsatisfactory, 80 % were successfully expressed in soluble and active form with LEXSY.

Protein Origin Problems with other systems Reference
Hydroxynitrile Lyase (MeHNL) Cassava plant Insoluble  Dadashipour et al.[1]
Laminin-332 human Non-homogenous product  Phan et al.[2]
N-Acetyl Serotonin Methyl Transferase (ASMT) human Degradation  Ben-Abdallah et al.[3]
Proprotein Convertase 4 rat Low yield  Basak et al.[4]
Tissue Plasminogen Activator human Not folded, Hyperglycosylation  Hemayatkar et al.[5]

Table 1: Selection of proteins successfully expressed with LEXSY, where expression with other systems failed.

   (2) LEXSY – everything for cloning, expression strain selection, and cultivation

LEXSY Expression Kits
Convenient all-in-one expression kits allowing constitutive or inducible, intracellular or secretory expression of target proteins. This is the place to start if you are new to LEXSY.

LEXSY Strains & Cultivation Kits
The LEXSY expression hosts, as strains or complete ready-to-grow cultivation kits.

LEXSY Cultivation Media
Complex and synthetic media for optimal growth of LEXSY strains.

LEXSY Antibiotics
For efficient selection of recombinant strains.

LEXSY Expression Vectors
Available with four different selection markers

PCR and Cloning enzymes
All restriction enzymes for the pLEXSY cloning sites, ligases, polymerases etc.

Need further information?

Please check out the LEXSY section at the Jena Bioscience website!

Or contact us by email at: expression@jenabioscience.com.

[1] Dadashipour et al. (2011) Comparative expression of wild-type and highly soluble mutant His103Leu of hydroxynitrile lyase from Manihot esculenta in prokaryotic and eukaryotic expression systems. Protein Expr. Purif. 77:92.
[2] Phan et al. (2009) The production of recombinant human laminin-332 in a Leishmania tarentolae expression system. Protein Expr. Purif. 68:79.
[3] Ben-Abdallah et al. (2011) Production of soluble, active acetyl serotonin methyl transferase in Leishmania tarentolae. Protein Expr. Purif. 75:114.
[4] Basak et al. (2008) Recombinant proprotein convertase 4 (PC4) from Leishmania tarentolae expression system: Purification, biochemical study and inhibitor design. Protein Expr. Purif. 60:117.
[5] Hemayatkar et al. (2010) Increased expression of recombinant human tissue plasminogen activator in Leishmania tarentolae. Biotechnol. J. 5:1198.

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