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Elucidating protein glycosylation with LEXSY


(1) LEXSY allows investigation of glycosylation effects in a human protease

(2) LEXSY – all you need for cloning, expression strain selection and cultivation

   



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   (1) LEXSY allows investigation of glycosylation effects in a human protease
   


Glycosylation – found in 50 % of all mammalian proteins – is a particularly important feature in human Kallikreins[1]. The Kallikrein-related Peptidase 2 (KLK2), which plays an important role in prostate cancer, has now been successfully expressed in glycosylated, homogenous and active form with LEXSY (the L. tarentolae based expression system)[2]. This allowed for the first time to study the effect of glycosylation on the activity of this key protein, showing that LEXSY is the expression system of choice for glycosylated proteins.

Figure 1 – Left: Purified KLK2 and detection of glycosylation by removal with PNGase F. The glycosylated protein is very homogenous and does not show double bands.
Right: Model of human KLK2. The glycan residue (blue and green) is a significant part of the protein and has a substantial effect on its activity. From   Guo et al., 2016[2].


   
   (2) LEXSY – all you need for cloning, expression strain selection, and cultivation
   


LEXSY Expression Kits
Convenient all-in-one expression kits allowing constitutive or inducible, intracellular or secretory expression of target proteins

LEXSY Strains & Cultivation Kits
The LEXSY expression hosts, as strains or complete ready-to-grow cultivation kits

LEXSY Culturing Media
Complex media for optimal growth of LEXSY strains

LEXSY Antibiotics
For efficient selection of recombinant strains

LEXSY Molecular Tools
Available with four different selection markers

PCR and Cloning enzymes
All restriction enzymes for the pLEXSY cloning sites, ligases, polymerases etc.


Need further information?

Please check out the LEXSY section at the Jena Bioscience website!


Or contact us by email at: expression@jenabioscience.com.


References:
[1] Guo et al. (2014) Sweetened kallikrein-related peptidases (KLKs): glycan trees as potential regulators of activation and activity. Biol. Chem. 395:959.
[2] Guo et al. (2016) A Single Glycan at the 99-Loop of Human Kallikrein-related Peptidase 2 Regulates Activation and Enzymatic Activity. J. Biol. Chem. 291:593.

   
   
   
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