The efficiency of Copper (Cu(I))-catalyzed Azide-Alkyne Click reactions (CuAAC) strongly depends on the presence of Copper ions in the +1 oxidation state (Cu(I)).
For most bioconjugation applications the combination of CuSO4 as Copper catalyst source, sodium ascorbate as reduction reagent and a water-soluble Cu(I) stabilizing ligand, such as BTTAA or THPTA, is recommended[1-2] (Fig. 1). BTTAA may promote a higher reaction efficiency under some experimental conditions.
Our CuAAC Reaction Buffer Kits (Tab. 1) allow
- convenient reaction set-up
- fine-tuning of reaction conditions: no black box, fully disclosed reagents and concentrations
- cost efficient scale-up: individual components are available as well
Figure 1: Azide- and Alkyne-functionalized molecules are efficiently conjugated under aqueous conditions using CuSO4 as Copper catalyst source, sodium ascorbate as reduction reagent and a water-soluble Cu(I) stabilizing ligand, such as BTTAA or THPTA[1-2].
Table 1: Available CuAAC Reaction Buffer Kits.
Also check out our Alkyne-functionalized metabolites, such as 5-EU, O-Propargyl-puromycin or Propargylcholine!
 Hong et al. (2012) Analysis and Optimization of Copper-Catalyzed Azide-Alkyne Cycloaddition for Bioconjugation. Angew. Chem. Int. Ed. 48:9879.
 Besanceney-Webler et al. (2011) Increasing the Efficiacy of Bioorthogonal Click Reactions for Bioconjugation: A Comparative Study. Angew. Chem. Int. Ed. 50:8051.