home
Convenient CuAAC Reaction Set-Up

Reaction Buffer Kits & Individual Components
   



Please click here if this newsletter is not displayed properly! To contact us please DO NOT reply to this newsletter but send your inquiry to info@jenabioscience.com.


   
   


The efficiency of Copper (Cu(I))-catalyzed Azide-Alkyne Click reactions (CuAAC) strongly depends on the presence of Copper ions in the +1 oxidation state (Cu(I)).

For most bioconjugation applications the combination of CuSO4 as Copper catalyst source, sodium ascorbate as reduction reagent and a water-soluble Cu(I) stabilizing ligand, such as BTTAA or THPTA, is recommended[1-2] (Fig. 1). BTTAA may promote a higher reaction efficiency under some experimental conditions[2].

Our CuAAC Reaction Buffer Kits (Tab. 1) allow

  • convenient reaction set-up
  • fine-tuning of reaction conditions: no black box, fully disclosed reagents and concentrations
  • cost efficient scale-up: individual components are available as well

Figure 1: Azide- and Alkyne-functionalized molecules are efficiently conjugated under aqueous conditions using CuSO4 as Copper catalyst source, sodium ascorbate as reduction reagent and a water-soluble Cu(I) stabilizing ligand, such as BTTAA or THPTA[1-2].


Product Cat. No.
CuAAC Biomolecule Reaction Buffer Kit (THPTA based) CLK-072
CuAAC Biomolecule Reaction Buffer Kit (BTTAA based) CLK-071
CuAAC Cell Reaction Buffer Kit (THPTA based) CLK-074
CuAAC Cell Reaction Buffer Kit (BTTAA based) CLK-073
CuAAC Reaction Ligand Test Kit (THPTA & BTTAA based) CLK-072

Table 1: Available CuAAC Reaction Buffer Kits.


Also check out our Alkyne-functionalized metabolites, such as 5-EU, O-Propargyl-puromycin or Propargylcholine!


Selected References:
[1] Hong et al. (2012) Analysis and Optimization of Copper-Catalyzed Azide-Alkyne Cycloaddition for Bioconjugation. Angew. Chem. Int. Ed. 48:9879.
[2] Besanceney-Webler et al. (2011) Increasing the Efficiacy of Bioorthogonal Click Reactions for Bioconjugation: A Comparative Study. Angew. Chem. Int. Ed. 50:8051.

   
   
   
   Responsible for content / Imprint
   
Jena Bioscience GmbH
Loebstedter Str. 71
07749 Jena
Germany

Phone: +49 – 3641 – 6285 000
Fax: +49 – 3641 – 6285 100
E-Mail: info@jenabioscience.com

Register Court: Amtsgericht Jena, HRB 207171
VAT No.: DE 195825742

        

Managing Directors:
Thomas Billert
Dr. Mathias Grün
   
    www.jenabioscience.com   
           
      © 2017 Jena Bioscience GmbH - Imprint