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Nourseothricin* and CRISPR/Cas

The genome engineering squad
   



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Nourseothricin*: Selection antibiotic of choice for CRISPR/Cas
   


Since its introduction in 2012, the CRISPR/Cas9 method has taken molecular biology labs worldwide by storm, making genome editing as simple as never before (Fig. 1)[1,2]. It requires just three components: The Cas9 nuclease, a target specific guide RNA and in most cases one or more antibiotics to select for clones expressing Cas9 and the guide RNA.

Nourseothricin has proven itself as a reliable antibiotic to ensure stable Cas9 or guide RNA expression especially in protista[3,4] and fungi[5-7]. A list with over 100 Nourseothricin-susceptible organisms can be found   here.

Figure 1: The principle of CRISPR/Cas. A specific guide RNA leads Cas9 to its target site in the genome, where it creates a double strand break which can be repaired by inserting the donor DNA of choice. A selection antibiotic like Nourseothricin is necessary to ensure stable Cas9 or guide RNA expression. From CRISPR-Cas: Extraordinary editing[8].


   

Nourseothricin* products at great prices
   


Nourseothricin is available as powder or sterile ready-to-go stock solution.

Product

Cat. No.

Amount

€ / g

Price (€)

Buy

Nourseothricin – powder

AB-102L

1 g

171,00

171,00

Nourseothricin – powder

AB-102XL

5 g

164,00

820,00

Nourseothricin – powder

AB-102-25G

25 g

144,80

3.620,00

Nourseothricin – powder

AB-102-100G

100 g

127,50

12.750,00

Product

Cat. No.

Amount

€ / ml

Price (€)

Buy

Nourseothricin – solution

AB-101S

1 ml

42,00

42,00

Nourseothricin – solution

AB-101L

5 ml

32,00

160,00

Nourseothricin – solution

AB-101-10ML

10 ml

30,00

300,00

Nourseothricin – solution

AB-101-50ML

50 ml

24,00

1.200,00

Or contact us by email at: expression@jenabioscience.com

* sometimes also termed NTC or ClonNat


References:
[1] Jinek et al. (2012) A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity. Science 337:816.
[2] Cong et al. (2013) Multiplex Genome Engineering Using CRISPR/Cas Systems. Science 339:819.
[3] Hopes et al. (2016) Editing of the urease gene by CRISPR-Cas in the diatom Thalassiosira pseudonana. Plant Methods 12:49.
[4] Poulsen et al. (2006) Molecular genetic manipulation of the diatom Thalassiosira pseudonana (Bacillariophyceae). J. Phycol. 42:1059.
[5] Vyas et al. (2015) A Candida albicans CRISPR system permits genetic engineering of essential genes and gene families. Sci. Adv. 1:e1500248.
[6] Generoso et al. (2016) Simplified CRISPR-Cas genome editing for Saccharomyces cerevisiae. J. Microbiol. Methods 127:203.
[7] Stovicek et al. (2015) CRISPR-Cas system enables fast and simple genome editing of industrial Saccharomyces cerevisiae strains. Metab. Eng. Commun. 2:13.
[8] Kåhrström et al. (2014) CRISPR-Cas: extraordinary editing. Nat. Rev. Microbiol. 12.

   
   
   
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