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Efficient Biomolecule Internalization

Cell Penetrating Peptides overcome cell membrane barriers
   



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Internalization of biomolecules such as proteins or nucleic acids into live cells is often hampered by the lack of biomolecule cell permeability. The use of Cell Penetrating Peptides (CPPs) as helper-molecules is a successful approach to overcome cell membrane barriers leading to efficient, low toxic biomolecule uptake into numerous cell lines both via covalent conjugate or non-covalent complex formation[1].

We now offer branched human calcitonine fragment hCT(18-32)-k7, a promising tool for efficient biomolecule internalization, that

  • allows convenient reaction set-up via non-covalent complex formation (Tab. 1)
  • shows only small cytotoxic effects[2]
  • is also available as fluorescent and biotinylated version for monitoring of uptake efficiency and intracellular distribution or complex formation with Avidin/Streptavidin-containing cargo, respectively (Tab. 2)


Table 1: hCT(18-32)-k7-mediated biomolecule internalization via non-covalent complex formation:


Application Cell lines Ref.
hCT(18-32)-k7 Nucleic acid internalization
Protein internalization
HEK293, MCF-7, COS-7, HeLa
HEK293T/17, polymorphonuclear leukocytes
[2,3]
[4]


Table 2: Overview of available hCT(18-32)-k7 versions:


Label Cat. No. Amount Price
hCT(18-32)-k7
CPP-P10 0.5 mg 150 €
Carboxyfluorescein CPP-P09 0.5 mg 180 €
Biotin CPP-P09 0.5 mg 180 €


Further CPPs such as sc18, CPPP-2, HIV-Tat(47-57) or Penetratin are available as well!

References:
[1] Reißmann (2014) Cell penetration: scope and limitations by the application of cell-penetrating peptides. J. Pept. Sci. 20:760.
[2] Rennert et al. (2008) Generation of carrier peptides for the delivery of nucleic acid drugs in primary cells. ChemMedChem 3.2:241.
[3] Hoyer et al. (2012) Knockdown of G protein-coupled receptor through efficient peptide-mediated siRNA delivery. J. Control. Rel. 161 (3):826.
[4] Leßig et al. (2013) Efficient inhibition of human leukocytic elastase by means of α1-antitrypsin/peptide complexes. Cytometry Part A 83.5:461.

   
   
   
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