from Cerrena unicolor
Enzyme Commission (EC) Number: 220.127.116.11
Benzenediol: oxygen oxidoreductase
For in vitro use only!
Unit Definition: One unit is defined as the amount of enzyme that oxidizes 1.0 μmole of ABTS [2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)] per minute at pH 4.5 and 25 °C.
Shipping: shipped on blue ice
Storage Conditions: store at -20 °C
Shelf Life: 12 months
EC number: 420-150-4
Form: Freeze-dried powder, no stabilizing agent added
A group of multi-copper proteins of low specificity acting on both o- and p-quinols, and often acting also on aminophenols and phenylenedia-mine. The semi-quinone may react further either enzymatically or non-enzymatically.
4 benzenediol + O2 → 4 benzosemiquinone + 2 H2O
Decolorization of dyes
Decompostion of phenolic and aromatic compounds
Optimal reaction with ABTS at pH 4.5, with Syringal-dazine at pH 5.0, Temperature range 15 - 50 °C
ABTS, Syringaldazine, 2,6-dimethoxyphenol
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