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from Cerrena unicolor

Enzyme Commission (EC) Number:

Benzenediol: oxygen oxidoreductase

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For in vitro use only!

Unit Definition: One unit is defined as the amount of enzyme that oxidizes 1.0 μmole of ABTS [2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)] per minute at pH 4.5 and 25 °C.

Shipping: shipped on blue ice

Storage Conditions: store at -20 °C

Shelf Life: 12 months

CAS#: 80498-15-3

EC number: 420-150-4

Form: Freeze-dried powder, no stabilizing agent added

Decolorization of dyes
Decompostion of phenolic and aromatic compounds

A group of multi-copper proteins of low specificity acting on both o- and p-quinols, and often acting also on aminophenols and phenylenedia-mine. The semi-quinone may react further either enzymatically or non-enzymatically.

4 benzenediol + O2 → 4 benzosemiquinone + 2 H2O

Reaction conditions:
Optimal reaction with ABTS at pH 4.5, with Syringal-dazine at pH 5.0, Temperature range 15 - 50 °C

Possible substrates:
ABTS, Syringaldazine, 2,6-dimethoxyphenol

Selected References:
Nitheranont et al. (2011) Extracellular Laccase Produced by an Edible Basidiomycetous Mushroom, Grifola frondosa: Purification and Characterization. Bioscience, Biotechnology, and Biochemistry 75 (3):538.
Karimi et al. (2009) Color remediation of chemimechanical pulping effluent using combination of enzymatic treatment and Fenton reaction. Desalination 249 (2):870.
Lin et al. (2003) Production of laccase by Coriolus versicolor and its application in decolorization of dyestuffs: (I). Production of laccase by batch and repeated-batch processes. J. Environ. Sci. (China) 15 (1):1.
Bertrand et al. (2002) Crystal structure of a four-copper laccase complexed with an arylamine: insights into substrate recognition and correlation with kinetics. Biochemistry 41:7325.
Brown et al. (2002) Expression and chracterization of a recombi-nant multi-copper oxidase: laccase IV from Trametes versiocolor. Inorg. Chim. Acta 331:232.
Galhaup et al. (2002) Characterization of the major laccase isoenzyme from Trametes pubescens and regulation of its synthesis by metal ions. Microbiology 148:2159.
Johannes et al. (2000) Laccase activity tests and laccase inhibitors. J. Biotechnol. 78:193.
Salas et al. (1995) Properties of laccase isoenzymes produced by the basidiomycete Ceriporiopsis subvermispora. Biotechnol. Appl. Biochem. 21:323.
Rogalski et al. (1991) Affinity chromatography: a convenient method for purification of fungal lactases. Biotechnol. Bioeng. 37:770.
Roy-Arcand et al. (1991) Direct dechlorination of chlorophenolic compounds by laccases from Trametes (Coriolus) versicolor. Enzyme Microb. Technol. 13:194.
Rogalski et al. (1990) Purification and immobilization of the inducible form of extracellular laccase of the fungus Trametes versicolor. Acta Biotechnol. 10:261.