from Nematoloma frowardii
Enzyme Commission (EC) Number: 188.8.131.52
Mn(II): hydrogen-peroxide oxidoreductase
For in vitro use only!
Unit Definition: One unit is defined as the amount of enzyme that oxidizes 1.0 μmole of Mn(II) per minute at pH 4.5 and 25 °C.
Shipping: shipped on blue ice
Storage Conditions: store at -20 °C
Shelf Life: 12 months
Form: Freeze-dried brown-coloured amorphous powder, no stabilizing agent added
Manganese Peroxidase is a hemoprotein involved in the oxidative degradation of lignin in white rot basidiomycetes. It catalyzes the oxidation of Mn(II) to Mn(III) by H2O2. The highly reactive Mn(III) is stabilized via chelation in the presence of dicarboxylic acid:
2 Mn(II) + 2 H+ + H2O2 → 2 Mn(III) + 2 H2O
Optimal reaction at pH 4.5, Temperature range 15 - 60 °C
Fournier et al. (2006) Degradation of CL-20 by white-rot fungi. Chemosphere 63:175.
Frigon et al. (2011) Impact of mechanical, chemical and enzymatic pre-treatments on the methane yield from the anaerobic digestion of switchgrass. Biomass and Bioenergy 36:1.