In-Meso Plate

LCP crystallization is the most popular method for membrane protein structure determination. Experiments are typically done in batch format. However, it is well known from the crystallization of soluble proteins that vapor diffusion can be a lot more powerful. The controlled in meso phase (CIMP) method combines the benefits of LCP and vapor diffusion to push membrane protein crystallization to new limits [1]. The patent-pending In-Meso Crystallization Plate provides an easy start with this revolutionary method.

Advantages

• Fully automatable method, compatible with any nanoliter robot
• Combines LCP with vapor diffusion to increase success rate
• Requires low protein concentrations (2-5 mg/ml)
• Plate is made from an advanced polymer and is therefore suitable for use under UV and polarized light

Several in meso phases can be reached with the CIMP technology, crystal formation can happen in any of these phases:

• Hydrated LCP phase: a modified lipidic cubic phase with water contents between 90 and 45%.
• Lipidic cubic phase (LCP): the classic in meso phase with water contents between 45 and 26%. Batch experiments typically are set up at water contents between 30 and 35%.
• Lamellar phase: characterized by increased contact between proteins. Water content below 26%.

Relationship between dilution of precipitant solution in the protein well, water content of the equilibrated protein drop, and the in meso phase that can be reached in a given experiment. Adapted from [1].

[1] Kubicek et al. (2012) Controlled In Meso Phase Crystallization - A Method for the Structural Investigation of Membrane Proteins. PLoS ONE 7:e35458.