Crystal Screens - JBScreen Membrane

JBScreen bulk & HTS

JBScreen Membrane covers 96 of the most successful conditions for crystallization of membrane proteins. Each individual composition results from an extensive analysis of the crystallization conditions that have yielded membrane protein structures so far.

The JBScreen Membrane crystallization conditions are primarily ordered by type and concentration of the precipitant. This allows easy extraction of all relevant information for a straightforward refinement: Once you get a hit, you immediately see the effects of the neighbouring conditions. The subsequent fine tuning of preliminary hits will be much more efficient.

JBScreen Detergents perfectly complement JBScreen Membrane: This combination enables you to screen a broad range of detergents, while concentrating on the most successful crystallization conditions, making crystallization screening of membrane proteins much more efficient and less time consuming.

Format

Bulk – 24 or 96 screening solutions in 10 ml aliquots
HTS – 96 screening solutions delivered in a deep-well block, 1.7 ml per well

 

Individual Conditions of all screens are available in 10 ml as well as 100 ml volumes.


Selected Literature Citations of JBScreen Membrane

  • Tan et al. (2014) A conformational landscape for alginate secretion across the outer membrane of Pseudomonas aeruginosa. Acta Cryst. D 70:2054.
  • Li et al. (2014) Crystallizing Membrane Proteins in the Lipidic Mesophase. Experience with Human Prostaglandin E2 Synthase 1 and an Evolving Strategy. Crystal Growth & Design 14:2034.
  • Jacobs et al. (2012) Expression, purification and crystallization of the outer membrane lipoprotein GumB from Xanthomonas campestris. Acta Cryst. F 68:1255.
  • Li et al. (2011) Crystallizing Membrane Proteins in Lipidic Mesophases. A Host Lipid Screen. Crystal Growth & Design 11(2):530.
  • Shaw Stewart et al. (2011) Random Microseeding: A Theoretical and Practical Exploration of Seed Stability and Seeding Techniques for Successful Protein Crystallization. Crystal Growth & Design 11(8):3432.
  • Caffrey et al. (2009) Crystallizing Membrane Proteins Using Lipidic Mesophases. Nat Protoc. 4:706.
  • Cherezov et al. (2006) In Meso Structure of the Cobalamin Transporter, BtuB, at 1.95 Å Resolution. J. Mol. Biol. 364:716.