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Phasing Kits - JBS Tungsten Cluster Derivatization Kits

Application

Heavy atom derivatization of biological macromolecules for isomorphous and/or anomalous phasing methods.
Experimental Tungsten (W) Phasing – either by SAD/MAD or MIR experiments – has become increasingly popular due to the high electron-density and good solubility in aqueous solutions of Polyoxotungstate Clusters.

The Tungstate Cluster Kits consist of 6 ready-to-use aliquots of Phosphotungstate, Metatungstate or Paratungstate salts, respectively. All Tungsten clusters contain 12 Tungsten metal centers bridged by Oxygen atoms, but differ in their resulting negative charge (3-, 6- and 10-, respectively).


Video Metatungstate Cluster still
Metatungstate-cluster bound to a positively charged surface patch of the death receptor 6 (DR6) (Dahms et al., 2013).

Move the mouse pointer over the image to start the animation.


References

  • Rudenko et al. (2003) 'MAD'ly phasing the extracellular domain of the LDL receptor: a medium-sized protein, large tungsten clusters and multiple non-isomorphous crystals. Acta Cryst. D59:1978.

Selected Literature Citations of JBS Tungsten Cluster Derivatization Kits

  • Dahms et al. (2013) Localization and orientation of heavy-atom cluster compounds in protein crystals using molecular replacement. Acta Cryst. D69:284.