Phasing Kits - JBS Magic Triangle

JBS Magic Triangle

JBS Magic Triangle is a phasing kit developed in co-operation with Tobias Beck in the research group of Prof. George M. Sheldrick, Georg-August University Göttingen.

The “Magic Triangle” I3C consists of three iodine atoms forming an equilateral triangle with a side length of 6.0 Å that can readily be identified in the electron density map.
It has been demonstrated for heavy-atom derivatization of macromolecules, and experimental phases have been derived using single-wavelength anomalous dispersion (SAD) or single isomorphous replacement plus anomalous scattering (SIRAS) methods.


References

  • Beck et al. (2009) How to get the magic triangle and the MAD triangle into your protein crystal. Acta Cryst. F65:1068.
  • Beck et al. (2008) A magic triangle for experimental phasing of macromolecules. Acta Cryst. D64:1179.
  • Sippel et al. (2008) Structure determination of the cancer-associated Mycoplasma hyorhinis protein Mh-p37. Acta Cryst. D64:1172.

Selected Literature Citations of JBS Magic Triangle

  • Benjdia et al. (2012) Structural insights into recognition and repair of UV-DNA damage by Spore Photoproduct Lyase, a radical SAM enzyme. Nucleic Acids Research 40:9308.