JBScreen Solubility HTS, developed by Meindert Lamers from the MRC in Cambridge, is designed to quickly find suitable buffer components to purify and store protein in.
JBScreen Solubility HTS tests for buffer, pH, salt and glycerol at the same time: For all proteins investigated, suitable conditions were found in a single assay. Protein and buffer conditions are dispensed in a ratio of 1:3, thereby minimizing the effect of any buffer components in which the protein is initially stored. Standard crystallization robots set up the assay in 5 minutes and very small amounts of protein are required (10 µl @ 5-10 mg/ml). The results are visible after one hour at high protein concentration or 12 hours at low protein concentration:
Examples of a drop without protein precipitation (left) and a drop with protein precipitation (right)