XP Screen

For general laboratory use.

Shipping: shipped at ambient temperature

Storage Conditions: store at 8-10 °C

Shelf Life: 12 months

Description:
The XP Screen provides 96 of the most prominent crystallization conditions upgraded with the Anderson−Evans polyoxotungstate [TeW₆O₂₄]⁶⁻ (TEW) at 1 mM final concentration. This additive has proven to promote crystal contacts, improve crystal quality and further provides a valuable anomalous signal for phasing due to 6 tungsten atoms^[1].
Optimized for TEW stability, the XP Screen improves crystallization and crystal diffraction quality of challenging targets.

Selected Literature Citations of XP Screen

• Sobala et al. (2020) Structure of human endo-α-1,2-mannosidase (MANEA), an antiviral host-glycosylation target. PNAS 117 (47):29595.
• Ames et al. (2020) Identifying a Molecular Mechanism That Imparts Species-Specific Toxicity to YoeB Toxins. Front Microbiol 11:959.

Related products:

• XP Up Screen, #CS-351
• Anderson-Evans polyoxotungstate (TEW), #X-TEW

BIOZ Product Citations:

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Selected References:
[1] Bijelic et al. (2017) Ten Good Reasons for the Use of the Tellurium-Centered Anderson-Evans Polyoxotungstate in Protein Crystallography. Acc. Chem. Res. 50:1441.
[2] Molitor et al. (2016) Aurone synthase is a catechol oxidase with hydroxylase activity and provides insights into the mechanism of plant polyphenol oxidases. Proc. Natl. Acad. Sci. 113:E1806.
[3] Molitor et al. (2016) In situ formation of the first proteinogenically functionalized [TeW₆O₂₄O₂(Glu)]^7- structure reveals unprecedented chemical and geometrical features of the Anderson-type cluster. Chem. Commun. 52:12286.
[4] Molitor et al. (2015) Crystallization and preliminary crystallographic analysis of latent, active and recombinantly expressed aurone synthase, a polyphenol oxidase, from Coreopsis grandiflora. Acta Cryst. F 71:746.
[5] Bijelic et al. (2015) Hen Egg-White Lysozyme Crystallisation: Protein Stacking and Structure Stability Enhanced by a Tellurium(VI)-Centred Polyoxotungstate. ChemBioChem 16:233.
[6] Mauracher et al. (2014) Latent and active abPPO4 mushroom tyrosinase cocrystallized with hexatungstotellurate(VI) in a single crystal. Acta Cryst. D 70:2301.
[7] Mauracher et al. (2014) Crystallization and preliminary X-ray crystallographic analysis of latent isoform PPO4 mushroom (Agaricus bisporus) tyrosinase. Acta Cryst. F 70:263.