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Detergent Screens

JBScreen Detergents contain 4 x 24 unique detergents that are compatible with most common crystallization reagents and are therefore perfectly suited for membrane protein solubilization:

  • Ionic detergents
  • Non-ionic detergents
  • Zwitterionic detergents
  • Non-detergent Sulfobetaines
  • Synthetic Lipids

JBScreen Detergents can be used throughout the protein purification process or can be added afterwards by dialysis or ion-exchange chromatography (detergent exchange). Detergent exchange can be vital for obtaining well-diffracting membrane-protein crystals [1].
JBScreen Detergents is also valuable for additive screening with detergents and detergent mixtures [2,3] in combination with the JBScreen Membrane. This combination will enable you to screen a broad range of detergents, while concentrating on the most successful crystallization conditions, making crystallization screening of membrane proteins much more efficient and less time consuming.


JBScreen Detergents & Prometheus: A winning team

Screen for stabilizing detergents by combining JBScreen Detergents with Prometheus NT.48, a label-free nanoDSF technology developed by NanoTemper Technologies, ideally suited for membrane protein stabilization.

 

BIOZ Product Citations

Please click the arrow on the right to expand the citation list. Click publication title for the full text.


References

[1] Rosenow et al. (2003) The influence of detergents and amphiphiles on the solubility of the light harvesting complex. Acta Cryst. D59:1422
[2] Adir (1999) Crystallization of the oxygen-evolving reaction centre of photosystem II in nine different detergent mixtures. Acta Cryst. D55:891
[3] Koronakis et al. (2000) Crystal structure of the bacterial membrane protein TolC central to multidrug efflux and protein export. Nature 405:914

Selected Literature Citation of JBScreen Detergents

  • Hofmann et al. (2020) High-Level Expression, Purification and Initial Characterization of Recombinant Arabidopsis Histidine Kinase AHK1. Plants 9(3):304.
  • Delle Bovi et al. (2017) Expression and purification of functional insulin and insulin-like growth factor 1 holoreceptors from mammalian cells. Anal. Biochem. DOI 10.1016/j.ab.2017.08.011.