Set of JBS Deep Purple, JBS True Blue, JBS Xtal Green and JBS Bright Red
Available again from January 2026
| Cat. No. | Amount | Price (EUR) | Buy / Note |
|---|---|---|---|
| CO-305 | 4 x 300 μl | 65,10 | Add to Basket/Quote Add to Notepad |
For general laboratory use.
Shipping: shipped at ambient temperature
Storage Conditions: store at ambient temperature
Shelf Life: 12 months
JBS Deep Purple
Crystal Violet
Cat. No.: CO-302
Volume: 300 μl
Molecular Formula: C25H30N3Cl
Molecular Weight: 407.98 g/mol
CAS#: 548-62-9
Concentration: 0.5 mM
JBS True Blue
Methylene Blue
Cat. No.: CO-301
Volume: 300 μl
Molecular Formula: C16H18ClN3S * H2O
Molecular Weight: 319.85 g/mol (anhydrous)
CAS#: 61-73-4
Concentration: 4 mM
JBS Xtal Green
Methylene Green
Cat. No.: CO-303
Volume: 300 μl
Molecular Formula: C16H17ClN4O2S * 0.5 ZnCl2
Molecular Weight: 433.00 g/mol
CAS#: 224967-52-6
Concentration: 2 mM
JBS Bright Red
Eosin Scarlet
Cat. No.: CO-304
Volume: 300 μl
Molecular Formula: C20H6Br2N2Na2O9
Molecular Weight: 624.08 g/mol
CAS#: 548-24-3
Concentration: 1 mM
Applications:
JBS crystal dye solutions are used to stain macromolecular crystals, i.e. protein, peptide and nucleic acid crystals in order to differentiate them from small molecules and salt crystals.
Description:
Crystallization screening with high concentrations of precipitant and salt may lead to the formation of salt crystals. It is quite difficult to make a distinction between these false positives and true protein crystals.
Staining of crystals with appropriate dyes is a very straightforward method to differentiate between macromolecular crystals and salt crystals.
Protein and salt crystals differ substantially in their solvent content. Small crystal dyes, like JBS Deep Purple, are able to permeate the solvent channels of a protein, thus coloring the protein purple. In contrast, salt crystals are tightly packed and do not possess large solvent channels. They will therefore remain colourless.
Usage:
Simply add 0.5-1 μl of JBS crystal dye solution to the crystallization drop containing the crystals of interest.
Coloring Time:
JBS Deep Purple colors protein crystals after a few minutes. Even if the color of the solution is only faintly purple under the microscope, proteins will be stained within 5-15 min.
Very occasionally, it has been reported that protein crystals did not absorb crystal dyes.
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