Crystallization Optimization: Input Protein

Get your protein ready for crystallization! Consider to alter the protein surface or modify the sequence by truncation or mutagenesis. Or try a new mode of production.

Existing Protein

• JBS Methylation Kit
  The 24-hour approach to protein surface engineering - modify crystal contacts by reductive methylation of Lysine residues
• JBS Floppy-Choppy
  In situ proteolysis as rescue technique in protein crystallization

Make a new / better Protein

• Random Mutagenesis Kits → Molecular Biology
  Within three billion years of evolution, nature has produced a plethora of proteins simply by repeated cycles of random mutagenesis followed by in vivo selection for superior function of the encoded proteins.
• LEXSY – Eukaryotic protein expression in Leishmania tarentolae → LEXSY Expression
  All tools required for protein expression with LEXSY in research and development: expression kits, LEXSY host, cultivation kits and tools, shuttle vectors and more. Leishmania tarentolae, isolated from the Moorish gecko Tarentola mauritanica, is not pathogenic to mammalians and requires Biosafety level 1.

From gene to crystallization within two days – LEXSY cell-free protein production

Talk held at HEC 13, September 24, 2010