For in vitro use only!
Shipping: shipped at ambient temperature
Storage Conditions: store at 4 °C
Shelf Life: 12 months
Molecular Formula: C4H11NO3 * HCl
EC number: 214-684-5
Concentration: 1 M
JBScreen Buffer Kit - Tris-HCl is designed for convenient reproduction and optimization of crystallization conditions. The solutions can be used to reformulate conditions of the JBScreen family, e.g. JBScreen Classic, JBScreen Basic, JBScreen Cryo, (see below for an example) and other commercially available crystallization screens.
Furthermore, JBScreen Buffer Kit - Tris-HCl can be employed for the straightforward preparation of custom screen solutions for the refinement and optimization of initial crystallization conditions. The JBScreen Buffer Kit formulations will help to save time preparing accurate and high-quality reagents for the reproducible production of single protein crystals.
The JBScreen Buffer Kit - Tris-HCl contains ready-made buffer solutions with preset pH values. Each buffer is provided as a 1.0 M stock solution and supplied in 10 ml volumes.
The JBScreen Buffer Kit - Tris-HCl comprises 20 reagents, covering a pH range from 7.1 - 9.0 in 0.1 pH unit increments. Each Tris-HCl solution has been carefully titrated using hydrochloric acid.
pKa-Value (20°C): 8.30
Useful pH-Range: 7.1 - 9.0
Titrated with: Hydrochloric Acid
JBScreen Classic 7, condition A2 (= B10 in JBScreen Classic HTS II):
12 % w/v MPD, 100 mM Tris-HCl pH 8.5, 50 mM Magnesium Chloride
Required stock solutions:
100 % w/v MPD, 1.0 M Tris-HCl pH 8.5 (JBScreen Buffer Kit),
2.0 M Magnesium Chloride
In order to make up 1 ml of a reservoir solution pipette
into the plate reservoir. Mix thoroughly until the solution is homogeneous.
Note: The final pH of the reservoir solution may vary from the indicated pH, depending on the nature of the reagents added to the JBScreen Buffer Kit solution.
For optimal results in the crystallization strategy, it is recommended to use the JBScreen Buffer Kit - Tris-HCl together with JBScreen Single Stocks.