Anderson-Evans polyoxotungstate

Polyhedral (A) and ball and stick (B) representation of [TeW₆O₂₄]⁶⁻ with different coordination modes of the oxygen atoms in panel B.^[1] Figure used by courtesy of Prof. Annette Rompel, University of Vienna.

For general laboratory use.

Shipping: shipped at ambient temperature

Storage Conditions: store at ambient temperature

Shelf Life: 12 months

Molecular Formula: Na₆[TeW₆O₂₄] x 22 H₂O

Molecular Weight: 2148.90 g/mol

Solubility: 100 mM in water Dissolve 20 mg in 93 μl water to achieve a 100 mM stock solution.

Description:
The Anderson−Evans polyoxotungstate (TEW) [TeW₆O₂₄]⁶⁻ is a universal and flexible additive for protein crystallization. With its planar structure and high negative charge, it has proven to promote crystal contacts, improve crystal quality and further provides a valuable anomalous signal for phasing due to 6 tungsten atoms.

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Selected References:
[1] Bijelic et al. (2017) Ten Good Reasons for the Use of the Tellurium-Centered Anderson-Evans Polyoxotungstate in Protein Crystallography. Acc. Chem. Res. 50:1441.
[2] Molitor et al. (2016) Aurone synthase is a catechol oxidase with hydroxylase activity and provides insights into the mechanism of plant polyphenol oxidases. Proc. Natl. Acad. Sci. 113:E1806.
[3] Molitor et al. (2016) In situ formation of the first proteinogenically functionalized [TeW₆O₂₄O₂(Glu)]^7- structure reveals unprecedented chemical and geometrical features of the Anderson-type cluster. Chem. Commun. 52:12286.
[4] Molitor et al. (2015) Crystallization and preliminary crystallographic analysis of latent, active and recombinantly expressed aurone synthase, a polyphenol oxidase, from Coreopsis grandiflora. Acta Cryst. F 71:746.
[5] Bijelic et al. (2015) Hen Egg-White Lysozyme Crystallisation: Protein Stacking and Structure Stability Enhanced by a Tellurium(VI)-Centred Polyoxotungstate. ChemBioChem 16:233.
[6] Mauracher et al. (2014) Latent and active abPPO4 mushroom tyrosinase cocrystallized with hexatungstotellurate(VI) in a single crystal. Acta Cryst. D 70:2301.
[7] Mauracher et al. (2014) Crystallization and preliminary X-ray crystallographic analysis of latent isoform PPO4 mushroom (Agaricus bisporus) tyrosinase. Acta Cryst. F 70:263.