JBScreen Nuc-Pro
JBScreen Nuc-Pro is designed to screen for preliminary crystallization conditions of nucleic acids and protein-nucleic acid complexes.
The highly effective sparse matrix screen is based upon extensive screening of the PDB, with focus on entries by structural genomic initiatives, the BMCD and other protocols [1-3]. Reported crystallization conditions for various RNAs, DNAs as well as protein-nucleic acid complexes were compiled and analyzed for rate of recurrence.
The 96 selected conditions cover a variety of polymers, mono- and divalent metal ions, organics, alcohols and buffers of a pH range from 4.0 to 8.5. The classification of the reagents into individual kits is based upon the main precipitant, i.e. various molecular weight PEGs, Salts, alcohols (MPD and 2-Propanol).
All reagents are tested for the absence of nuclease contamination and are certified to be free of DNase and RNase.
Format
Bulk – 24 or 96 screening solutions in 10 ml aliquots
HTS – 96 screening solutions delivered in a deep-well block, 1.7 ml per well
Products & Ordering
BIOZ Product Citations
Powered by BiozSelected Literature
- Dolot et al. (2021) Biochemical, crystallographic and biophysical characterization of histidine triad nucleotide-binding protein 2 with different ligands including a non-hydrolyzable analog of Ap4A. BBA - General Subjects 1865(11):129968.
- Alipov et al. (2021) Structure of a Mutant Form of Translation Regulator Hfq with the Extended Loop L4. Crystallography Reports 66:791.
- Kim et al. (2020) Ligand binding characteristics of the Ku80 von Willebrand domain. DNA Repair 85:102739.
- Yin et al. (2017) Impact of cytosine methylation on DNA binding specificities of human transcription factors. Science 356 eaaj2239.
- Nemchinova et al. (2017) An Experimental Tool to Estimate the Probability of a Nucleotide Presence in the Crystal Structures of the Nucleotide–Protein Complexes. Protein J DOI 10.1007/s10930-017-9709-y.
- Wang et al. (2016) Base pairing and structural insights into the 5-formylcytosine in RNA duplex. Nucleic Acids Research 44:4968.
- Nikulin et al. (2016) Characterization of RNA-binding properties of the archaeal Hfq-like protein from Methanococcus jannaschii. J Biomol Struct Dyn DOI:10.1080/07391102.2016.1189849.
- Morgunova et al. (2015) Structural insights into the DNA-binding specificity of E2F family transcription factors. Nat. Commun. DOI:10.1038/ncomms10050.
- Tishchenko et al. (2013) Crystallization and preliminary X-ray diffraction studies of Drosophila melanogaster Gao-subunit of heterotrimeric G protein in complex with the RGS domain of CG5036. Acta Cryst. F 69:61.
References and Recommended Reading:
[1] Doudna et al. (1993) Crystallization of ribozymes and small RNA motifs by a sparse matrix approach. Proc. Natl. Sci. USA 90:7829.
[2] Scott et al. (1995) Rapid Crystallization of Chemically Synthesized Hammerhead RNAs using a Double Screening Procedure. J. Mol. Biol. 250:327.
[3] Ke et al. (2004) Crystallization of RNA and RNA-protein complexes. Methods 34:408.
Contact
Dr. Buerk Schaefer
and for inquiries regarding Molecular Biology & Proteins