Cell-penetrating and cytoprotective pentapeptide
Cell penetrating peptide for transduction of peptides, proteins and nucleotides into live cells and for cytoprotection
For in vitro use only!
Shipping: shipped on blue ice
Storage Conditions: store at -20 °C
Shelf Life: 12 months after date of delivery
Molecular Weight: 605 Da confirmed by MALDI-MS.
Purity: > 95 % (HPLC)
Form: Synthetic peptide, water soluble powder, contains CF3COO- (trifluoro acetate) as counter ion.
Activity: 1 μl of stock solution is able to form a non-covalent complex with 1 μg of a protein of MW of 100 kDa. For different MWs adjust amount of stock solution accordingly.
CPPP-2 is one of the cell penetrating pentapeptides (CPPPs) designed from Bax inhibiting peptides (BIPs). It is used for internalization of peptides and proteins (GFP) into different types of live cells. CPPP-2 uses yet unidentified mechanisms for cell penetration including mechanisms not requiring interaction with proteoglycans. The transport of cargo requires in some cases only formation of a non-covalent complex however, for most applications a conjugate with the cargo has to be formed and an excess of free peptide is added to improve internalization. The peptide shows some cytoprotective activity. It suppresses Bax-mediated apoptosis and is therefore recommended to protect cells from cytotoxic stress. CPPPs may be utilized for non-toxic drug delivery. The influence of CPPP-2 on cell viability is tested on different cell lines (including HeLa, Jurkat, Swiss 3T3, NIH 3T3, NB-4 and COS-7). For most of these cells it has no toxic effect up to a concentration of 20 μg/ml serum-free transduction medium. In many cell lines it even improves the viability. Thus, it can be widely used for internalization of proteins. But, it requires a molar ratio of about 1:100.
Peptide provides 2 positive charges for complex formation, 2 trifluoro acetate residues are present resulting in an apparent MW of about 0.9 kDa.
Dissolve 1.2 mg (1 vial) in 1 ml sterile and oxygen-free water according to the general manual. Use the solution immediately or aliquot and store at -20 °C. Avoid freeze / thaw cycles. Please note that the peptide may form S-oxide (Met) when stored in solution.
Perform calculation, complex formation and cargo transduction according to the detailed protocols given in the general manual.
Jena Bioscience Publications using CPPP-2:
Formation of non-covalent complexes with different cargos, transport into different cell lines, uptake efficiencies and cytotoxicity's are described in four publications:
Mussbach et al. (2011). Internalization of nucleoside phosphates into live cells by complex formation with different cell penetrating peptides and JBS-Nucleoducin. In: Langel U., Editor. Cell penetrating peptides Methods and Protocols. Methods in Molecular Biology, vol. 683, Humana Press, Springer, New York, Dordrecht, Heidelberg, London. pp. 375-389.
Mussbach et al. (2011). Transduction of peptides and proteins into live cells by cell penetrating peptides. J. Cell. Biochem. 112: 3824.
Keller et al. (2013). Relationships between cargo, cell penetrating peptides and cell type for uptake of non-covalent complexes into live cells. Pharmaceuticals 6: 184.
Keller et al. (2014). Transduction of proteins into Leishmania tarentolae by formation of non-covalent complexes with cell-penetrating peptides. J. Cell. Biochem. 115: 243.
Handbook of Cell-Penetrating Peptides, Second Edition, Ed. by Ü. Langel, CRC Taylor and Francis, Boca Raton, London, New York (2007).
Cell-Penetrating Peptides, Methods and Protocolls, Edited by Ülo Langel, Methods in Molecular Biology 683, Springer New York, Dodrecht, Heidelberg, London (2011).
Pharmaceuticals, Special Issue 'Cell penetrating Peptides' (2010-2013).
Morris et al. (2008). Cell-penetrating peptides: from molecular mechanism to therapeutics. Biology of the Cell 100: 201.
Gros et al. (2006) A non-covalent peptide-based strategy for protein and peptide nucleic acid transduction. Biochim. Biophys. Acta 1758:384.
Gomez et al. (2007) Bax-inhibiting peptides derived from Ku70 and cell-penetrating pentapeptides. Biochem. Soc. Trans. 35:797.
Mussbach et al. (2011) Internalization of nucleoside phosphates into live cells by complex formation with different CPPs and JBS-Nucleoducin. Methods in Molecular Biology 683:375.
Gomez et al. (2010). Cell-penetrating penta-peptides (CPP5s): Measurement of cell entry and protein transduction activity. Pharmaceuticals 3: 3594.
Availability Restriction: Exclusively distributed in Japan by Greiner BioOne