Cocktail for transduction of nucleotides into live cells
For in vitro use only!
Shipping: shipped on blue ice
Storage Conditions: store at -20 °C
Shelf Life: 12 months
Form: lyophilized powder, contains CF3COO- (trifluoro acetate) as counter ion
Solubility: soluble in water
Activity: 4 μl of stock solution is necessary for formation of a non-covalent complex with 1 μg of a nucleoside triphosphate (MW approx. 0.5 kDa carrying 4 negative charges). For different MWs and/or different charges adjust amount of stock solution accordingly.
Availability Restriction: Exclusively distributed in Japan by Greiner BioOne
JBS-Nucleoducin is a mixture of different cell penetrating peptides and proteins, optimized for internalization of nucleoside phosphates into living cells. Some components contain a nuclear localization sequence.
Compared to other CPP or lipid transduction reagents the transduction rate and efficiency are strongly enhanced.
The multi-component composition of JBS-Nucleoducin
Dissolve the whole content of the vial in sterile and oxygen free water (CPP-C02S in 250 μl; CPP-C02L in 1.25 ml) according to the general manual. Use the solution immediately or aliquot and store at -20 °C. Please note that the cocktail has proteolytic activity and may form disulfides and S-oxide (Met) when stored in solution. Please also note that the cocktail may form insoluble disulfides (Cys) and S-oxides (Met) when stored in solution. For elimination of potential precipitated material quick-spin stock solution (5.000 - 10.000 g, 5 min) immediately prior use and transfer supernatant into a fresh tube.
Perform calculation, complex formation and cargo transduction according to detailed protocols given in the general manual. JBS-Nucleoducin can be used for transduction of larger amounts.
Handbook of Cell-Penetrating Peptides, Second Edition, Ed. by Ü. Langel, CRC Taylor and Francis, Boca Raton, London, New York (2007).
Morris et al. (2008) Cell penetrating peptides: from molecular mechanisms to therapeutics. Bio.Cell. 100:201.
Ziegler et al. (2007) High affinity of the cell-penetrating peptide HIV-1 Tat-PTD for DNA. Biochemistry. 46:8138.
Turner et al. (2006) Targeting the HIV-1 RNA leader sequence with synthetic oligonucleotides and siRNA: chemistry and cell delivery.
Biochim. Biophys. Acta.1758:290.
Abes et al. (2007) Peptide-based delivery of nucleic acids: design, mechanism of uptake and applications to splice-correcting oligonucleotides. Biochem. Soc. Trans. 35:53.
Gros et al. (2006) A non-covalent peptide-based strategy for protein and peptide nucleic acid transduction. Biochim. Biophys. Acta 1758:384.
Simeoni et al. (2005) Peptide-based strategy for siRNA delivery into mammalian cells. Methods. Mol. Biol. 309:251.
Rubio et al. (2004) Quantification of absolute Ras-GDP/GTP levels by HPLC separation of Ras-bound [ (32)P]-labelled nucleotides.
J. Biochem. Biophys. Methods 58:111.
Mussbach et al. (2011) Internalization of nucleoside phosphates into live cells by complex formation with different CPPs and JBS-Nucleoducin. Methods in Molecular Biology 683:375.