JBS-Nucleoducin

Cocktail for transduction of nucleotides into live cells

Product Cat. No. Amount Price (EUR) Buy / Note
S pack CPP-C02S 75 reactions 130,00 Add to Basket/Quote Add to Notepad
L pack CPP-C02L 375 reactions 520,00 Add to Basket/Quote Add to Notepad

For in vitro use only!

Shipping: shipped on blue ice

Storage Conditions: store at -20 °C

Shelf Life: 12 months

Form: lyophilized powder, contains CF3COO- (trifluoro acetate) as counter ion

Solubility: soluble in water

Activity: 4 μl of stock solution is necessary for formation of a non-covalent complex with 1 μg of a nucleoside triphosphate (MW approx. 0.5 kDa carrying 4 negative charges). For different MWs and/or different charges adjust amount of stock solution accordingly.

Availability Restriction: Exclusively distributed in Japan by Greiner BioOne

Description:
JBS-Nucleoducin is a mixture of different cell penetrating peptides and proteins, optimized for internalization of nucleoside phosphates into living cells. Some components contain a nuclear localization sequence.
Compared to other CPP or lipid transduction reagents the transduction rate and efficiency are strongly enhanced.
The multi-component composition of JBS-Nucleoducin

  • facilitates efficient complexation with nucleotide cargos
  • is compatible with a large number of cell types and membrane structures
  • triggers different transduction mechanisms
JBS-Nucleoducin shows only marginal cytotoxic effects on a number of cell lines (including HeLa, Jurkat, Swiss 3T3, NIH 3T3, NB-4, and COS-7) and can be therefore, widely used for internalization of nucleotides. For cell survival the critical concentration of JBS-Nucleoducin in serum-free transduction medium is in the range of 10 % stock solution (20 μl in 200 μl) at which cell viability and cell membrane integrity are only marginally reduced (approx. 30 %).

Stock solution:
Dissolve the whole content of the vial in sterile and oxygen free water (CPP-C02S in 250 μl; CPP-C02L in 1.25 ml) according to the general manual. Use the solution immediately or aliquot and store at -20 °C. Please note that the cocktail has proteolytic activity and may form disulfides and S-oxide (Met) when stored in solution. Please also note that the cocktail may form insoluble disulfides (Cys) and S-oxides (Met) when stored in solution. For elimination of potential precipitated material quick-spin stock solution (5.000 - 10.000 g, 5 min) immediately prior use and transfer supernatant into a fresh tube.

Usage:

Perform calculation, complex formation and cargo transduction according to detailed protocols given in the general manual. JBS-Nucleoducin can be used for transduction of larger amounts.

Selected References:
Handbook of Cell-Penetrating Peptides, Second Edition, Ed. by Ü. Langel, CRC Taylor and Francis, Boca Raton, London, New York (2007).
Morris et al. (2008) Cell penetrating peptides: from molecular mechanisms to therapeutics. Bio.Cell. 100:201.
Ziegler et al. (2007) High affinity of the cell-penetrating peptide HIV-1 Tat-PTD for DNA. Biochemistry. 46:8138.
Turner et al. (2006) Targeting the HIV-1 RNA leader sequence with synthetic oligonucleotides and siRNA: chemistry and cell delivery.
Biochim. Biophys. Acta.1758:290.
Abes et al. (2007) Peptide-based delivery of nucleic acids: design, mechanism of uptake and applications to splice-correcting oligonucleotides. Biochem. Soc. Trans. 35:53.
Gros et al. (2006) A non-covalent peptide-based strategy for protein and peptide nucleic acid transduction. Biochim. Biophys. Acta 1758:384.
Simeoni et al. (2005) Peptide-based strategy for siRNA delivery into mammalian cells. Methods. Mol. Biol. 309:251.
Rubio et al. (2004) Quantification of absolute Ras-GDP/GTP levels by HPLC separation of Ras-bound [ (32)P]-labelled nucleotides.
J. Biochem. Biophys. Methods 58:111.
Mussbach et al. (2011) Internalization of nucleoside phosphates into live cells by complex formation with different CPPs and JBS-Nucleoducin. Methods in Molecular Biology 683:375.