The ability to purify recombinant proteins using affinity chromatography has greatly advanced protein research.
In affinity chromatography, the protein of interest is purified by its ability to bind a specific ligand that is immobilized on a chromatographic bead material (matrix).
Crude cell-lysates are incubated with such a matrix under conditions that ensure specific binding of the protein to the immobilized ligand. Other proteins that do not bind the immobilized ligand are washed through. Elution of the bound protein of interest can be achieved by changing the experimental conditions to favour desorption.
Jena Bioscience offers both Agarose and Magnetic Beads as matrix for different purification tasks.
Also available: Auxilary Equipment such as empty Columns, Spin Columns or Columns for FPLC and ÄktaTM design instruments.
Click on the links below to visit the appropriate product sections:
|Purification of||Agarose||Magnetic Beads|
|Nucleotide-binding proteins||NTP/NDP/NMP Agaroses|
|mRNA cap binding proteins||m7GTP Agarose|
|ATP-binding proteins||ATP Agaroses|
ATP Affinity Test Kit
ATP Affinity Purification Kits
|AMP-binding proteins||AMP Agaroses|
AMP Affinity Test Kit
|Phospho Amino Acid binding proteins||O-Phospho-Serine Agarose|
|GST-tagged proteins||Glutathione Agarose||Glutathione Magnetic Beads|
|His-tagged proteins||Ni-NTA Agarose|
Ni-, Zn-, Co-, Cu-IDA Agarose (gravity flow)
|Ni-NTA Magnetic Beads|
Co-NTA Magnetic Beads
Ni-IDA Magnetic Beads
Co-IDA Magnetic Beads
|Antibodies||Protein A Agarose|
mAB-Protein A Agarose (alkali-stable)
Protein G Agarose
Protein A/G Agarose
|CLICK-functionalized proteins||Azide Agarose, PC Azide Agarose|
Alkyne Agarose, PC Alkyne Agarose
|Azide Magnetic Beads|
Alkyne Magnetic Beads
DBCO Magnetic Beads