LEXSY Configurations - Vectors

The constitutive pLEXSY-2 vectors (EGE-231 to EGE-234) and inducible pLEXSY_I vectors (EGE-243 to EGE-251) were developed for both, intracellular or secretory expression of target proteins.

For constitutive expression we recommend the standard vector pLEXSY-sat2 for selection of LEXSY expression strains with the antibiotic Nourseothricin (NTC). For inducible expression we recommend the integrative vector pLEXSY_I-blecherry3 for selection of LEXSY expression strains with the antibiotic LEXSY Bleo and Cherry fluorescence monitoring of induction.

For expression plasmid construction we recommend our ligase-free CyClone technology → Molecular Biology in combination with our Primer Designer software (web shop account required)!

Control vectors and primers on request.