Want to detect His-tag fusion proteins
...in just 15 - 90 min time?
...with no need to apply a second antibody?
...without significant hands-on time?
Then read more below!
The His Detect Solution is a ready-to-use reagent for high speed detection of his-tagged proteins. It contains an optimized poly-histidine primary antibody conjugated to labeled gold nanoparticles for direct protein visualization. Staining occurs by simply adding the solution onto the blot and incubating for 15 to 90 min with NO need of second antibody. His-Detect is highly specific for C- and N-terminal His-tags and sensitive up to sub-picomole protein levels.
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 Cornvik et al. (2005): Colony filtration blot: a new screening method for soluble protein expression in Escherichia coli. Nat. Methods 2:507.