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His-tagged Proteins - Nickel NTA Agaroses

Suitable for FPLC, gravity flow and batch purification

His-tagged proteins are efficiently purified by a one-step procedure from crude lysates both under denaturing and non-denaturing conditions.

  • High capacity
  • Minimum metal leaching

Product characteristics

Matrix6 % cross-linked agarose
Bead size50-170 µm
LinkerNitrilotriacetic acid (NTA)
Binding capacity*> 50 mg/ml gel
Recommended flow rate1 ml column – 1.0 ml/min
10 ml column – 5.0 ml/min
Maximum linear flow rate300 cm/h
Maximum pressure3 bar (43.5 psi)
Recommended pHbetween 7 - 8
Chemical stabilityStable to all solutions commonly used in gel filtration including reducing agents (see procedure)

* Tested with 6xHis–GFPuv. Binding capacity will differ for each target protein.