DBCO Reagents - (Desthio) Biotinylation Reagents

Labeling of a molecule (e.g. a protein or nucleic acid) with biotin is routinely performed for its subsequent affinity purification via streptavidin agarose or detection with fluorescent or HRP-labeled streptavidin. Due to the extremely high affinity of biotin towards streptavidin (KD = 10-15 M), the biotinylated molecule/streptavidin-interaction is essentially irreversible under physiological conditions. Desthiobiotin however, binds less tightly to streptavidin (KD = 10-11 M) and desthiobiotinylated molecules are therefore easily eluted from the complex in the presence of excess Biotin.

Dibenzocyclooctyne (DBCO)*-containing (desthio) biotinylation reagents can be used for the labeling of Azide-tagged biomolecules via Cu(I)-free strain-promoted Alkyne-Azide Click Chemistry (SPAAC).

A number of (desthio) biotinylation reagents with

  • different solubility characteristics (PEG- and Sulfo-linker for increased molecule solubility)
  • different cleavability characteristics (chemically or photocleavable linke)
are available to fit specific application & molecule requirements.

*DBCO is also known as ADIBO (= Azadibenzocyclooctyne) or DIBAC (= Dibenzoazacyclooctyne)

 

Table 1: Available DBCO-containing (Desthio) Biotinylation Reagents

LabelDBCO
BiotinDBCO-PEG4-Biotin
Sulfo-DBCO-Biotin
Biotin with chemically cleavable linkerDiazo Biotin-DBCO
Dde Biotin-DBCO
Biotin with photocleavable linkerPhotocleavable Biotin-DBCO
Photocleavable Biotin-Sulfo-DBCO
DesthiobiotinDBCO-PEG4-Desthiobiotin