5-(Octa-1,7-diynyl)-2'-deoxycytidine 5'-triphosphate, Sodium salt
For research use only!
Shipping: shipped on blue ice
Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.
Shelf Life: 12 months after date of delivery
Molecular Formula: C17H24N3O13P3 (free acid)
Molecular Weight: 571.31 g/mol (free acid)
Purity: > 99 %
Form: clear aqueous solution
Concentration: 100 mM
Spectroscopic Properties: ε260 nm 5.0 L mmol-1 cm-1 (in water); ε292 nm 9.5 L mmol-1 cm-1 (in water)
Incorporation into DNA by PCR with family B polymerases (Pwo, Deep Vent exo- or KOD XL)[1,2]
Incorporation into DNA by Primer Extension with family A polymerase Taq and family B polymerases (Pwo, Deep Vent exo- or KOD XL)
Incorporation into DNA by 3'-End Labeling with Terminal deoxynucleotidyl Transferase(TdT)
The resulting ethynyl-functionalized DNA can subsequently be processed via Cu(I)-catalyzed click chemistry that offers the choice
 Gierlich et al. (2007) Synthesis of Highly Modified DNA by a Combination of PCR with Alkyne-Bearing Triphosphates and Click Chemistry. Chem. Eur. J. 13:9486.
 Burley et al. (2006) Directed DNA Metallization. J. Am. Chem. Soc. 128 (5):1398.
 Sorensen et al. (2013) Enzymatic Ligation of Large Biomolecules to DNA. ACSnano 7 (9):8098.
 Presolski et al. (2011) Copper-Catalyzed Azide-Alkyne Click Chemistry for Bioconjugation. Current Protocols in Chemical Biology 3:153.
 Hong et al. (2011) Analysis and Optimization of Copper-Catalyzed Azide-Alkyne Cycloaddition for Bioconjugation. Angew. Chem. Int. Ed. 48:9879.