C8-Alkyne-dUTP

5-(Octa-1,7-diynyl)-2'-deoxyuridine 5'-triphosphate, Sodium salt

Product Cat. No. Amount Price (EUR) Buy / Note
S pack CLK-T05-S 5 μl (100 mM) 125,76 Add to Basket/Quote Add to Notepad
L pack CLK-T05-L 5 x 5 μl (100 mM) 503,04 Add to Basket/Quote Add to Notepad
XL pack CLK-T05-XL 50 μl (100 mM) 754,56 Add to Basket/Quote Add to Notepad

For research use only!

Shipping: shipped on blue ice

Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible. If stored as recommended, Jena Bioscience guarantees optimal performance of this product for 12 months after date of delivery.

Shelf Life: 12 months

Molecular Formula: C17H23N2O14P3 (free acid)

Molecular Weight: 572.29 g/mol (free acid)

Purity: > 99 %

Form: clear aqueous solution

Concentration: 100 mM

pH: 7.5

Spectroscopic Properties: ε260 nm 3.9 L mmol-1 cm-1 (in water); ε292 nm 11.0 L mmol-1 cm-1 (in water)

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Applications:
Incorporation into DNA by PCR with family B polymerases (Pwo, Deep Vent exo- or KOD XL)[1,2]
Incorporation into DNA by Primer Extension with family A polymerase Taq and family B polymerases (Pwo, Deep Vent exo- or KOD XL)[1]
The resulting ethynyl-functionalized DNA can subsequently be processed via Cu(I)-catalyzed click chemistry that offers the choice

  • to introduce a Biotin group (via Azides of Biotin) for subsequent purification tasks
  • to introduce fluorescent group (via Azides of fluorescent dyes) for subsequent microscopic imaging
  • to crosslink the DNA to Azide-functionalized biomolecules e.g.proteins
Presolski et al.[3] and Hong et al.[4] provide a general protocol for Cu(I)-catalyzed click chemistry reactions that may be used as a starting point for the set up and optimization of individual assays.

Selected References:
[1] Gierlich et al. (2007) Synthesis of Highly Modified DNA by a Combination of PCR with Alkyne-Bearing Triphosphates and Click Chemistry. Chem. Eur. J. 13:9486.
[2] Burley et al. (2006) Directed DNA Metallization. J. Am. Chem. Soc. 128 (5):1398.
[3] Presolski et al. (2011) Copper-Catalyzed Azide-Alkyne Click Chemistry for Bioconjugation. Current Protocols in Chemical Biology 3:153.
[4] Hong et al. (2011) Analysis and Optimization of Copper-Catalyzed Azide-Alkyne Cycloaddition for Bioconjugation. Angew. Chem. Int. Ed. 48:9879.