L-Ascorbic acid sodium salt
For research use only!
Shipping: shipped at ambient temperature
Storage Conditions: store at ambient temperature
Shelf Life: 12 months
Molecular Formula: C6H7NaO6
Molecular Weight: 198.11 g/mol
Purity: ≥ 98 % (TLC)
Form: white powder
Na-Ascorbate can be used as a reduction reagent for Cu(I)-catalyzed Alkyne-Azide click chemistry reactions (CuAAC).
It catalyzes the reduction of Cu(II) sources such as CuSO4 thereby releasing catalytically reactive Cu(I) ions.
Ideally, solutions should be freshly prepared in ddH2O shortly before use. Alternatively, a stock solution can be prepared, stored at -20°C and freshly by diluted shortly before use.
Please note: Do not use solutions that appear brown. Freshly prepared, fully functional Na-Ascorbate solutions are colorless and turn brown upon oxidization thereby loosing their reduction capability.
Presolski et al. and Hong et al. provide a general protocol for CuAAC reactions that may be used as a starting point for the set up and optimization of individual assays.
 Presolski et al. (2011) Copper-Catalyzed Azide-Alkyne Click Chemistry for Bioconjugation. Current Protocols in Chemical Biology 3:153.
 Hong et al. (2011) Analysis and Optimization of Copper-Catalyzed Azide-Alkyne Cycloaddition for Bioconjugation. Angew. Chem. Int. Ed. 48:9879.