Abs/Em = 568/584 nm
For research use only!
Shipping: shipped at ambient temperature
Storage Conditions: store at -20 °C
Shelf Life: 12 months
Molecular Formula: C35H46N6O9S2
Molecular Weight: 758.90 g/mol
Purity: > 90 % (HPLC)
Form: dark red solid
Solubility: DMF, DMSO, MeOH
Spectroscopic Properties: λabs 568 nm; λem 585 nm; ε 91.0 L mmol-1 cm-1
The fluorescence emission spectrum of the Lissamine™ rhodamine B probe lies between those of tetramethylrhodamine and Texas Red probes. With peak absorption at 568 nm, Dibenzylcyclooctyne-Fluor 568 is readily excited by the 568 nm spectral line of the Ar - Kr mixed gas laser commonly used in many confocal laser-scanning microscopes. As many sulforhodamine probes, conjugates of Lissamine™ rhodamine B tend to exhibit higher fluorescence intensity than non-sulfonated rhodamine conjugates due to the higher negative charge of sulfonic groups compared to carboxyl groups. The fluorescence of the azide - Lissamine™ rhodamine B conjugate as well as of many other rhodamine based probes is pH insensitive between 4 and 9, and exhibits excellent photostability.