Abs/Em = 501/525 nm
For research use only!
Shipping: shipped at ambient temperature
Storage Conditions: store at -20 °C
Shelf Life: 12 months after date of delivery
Molecular Formula: C29H30N6O7
Molecular Weight: 574.59 g/mol
Purity: > 90 % (HPLC)
Form: dark red solid
Solubility: DMF, DMSO, MeOH
Spectroscopic Properties: λabs 501 nm; λem 525 nm; ε 74.0 L mmol-1 cm-1 (in MeOH)
With excitation maximum at 501 nm, the azide - carboxyrhodamine 110 conjugate is an excellent match to the intense 488 nm spectral line of the argon ion laser commonly used in many confocal laser-scanning microscopes. Carboxyrhodamine 110-PEG3-azide is an excellent green fluorescent rhodamine dye. The conjugate has better solubility than the parent dye carboxyrhodamine and its long PEG spacer should reduce any steric effect of the dye on the molecule to be labelled. Carboxyrhodamine 110 and its derivatives are, in general, significantly more photostable than any other known green fluorescent dyes including Alexa 488. In addition, the fluorescence of the azide-PEG3-carboxyrhodamine 110 conjugate is completely insensitive to pH between 4 and 9. Unlike Alexa 488, which is readily degraded under alkaline condition, carboxyrhodamine 110 and its derivatives are highly stable under both acidic and basic conditions. As a result, carboxyrhodamine 110 and its derivatives are superior alternatives to other green fluorescent dyes.
Shieh et al. (2014) Imaging bacterial peptidoglycan with near-infrared fluorogenic azide probes. Proc. Natl. Acad. Sci. USA 111 (15):5456.