For research use only!
Shipping: shipped at ambient temperature
Storage Conditions: store at -20 °C
Shelf Life: 12 months
Molecular Formula: C18H32N6O5S
Molecular Weight: 444.54 g/mol
Purity: > 95 % (H NMR)
Form: off-white to grey solid
Solubility: Chloroform, DCM, DMF, DMSO, THF
Azide-PEG3-Biotin is suitable for the introduction of a biotin moiety to terminal Alkyne- and strained Alkyne (e.g. DBCO)-labeled biomolecules via Cu(I)-catalyzed Alknye-Azide (CUAAC) or Cu(I)-free strain-promoted Alkyne-Azide Click Chemistry (SPAAC) reaction, respectively.
The hydrophilic PEG3 linker reduces or eliminates aggregation and precipitation during the labeling process by increasing the hydrophilicity of the target molecule. It furthermore enhances the accessibility of the biotin moiety and thus the detection efficiency of the biotinylated molecule via fluorescent or HRP-labeled streptavidin or its affinity purification via streptavidin agarose.
Due to the extremely high affinity of biotin towards streptavidin (KD = 10-15 M), the biotinylated molecule/streptavidin-interaction is essentially irreversible under physiological conditions.