For research use only!
Shipping: shipped at ambient temperature
Storage Conditions: store at -20 °C
Shelf Life: 12 months after date of delivery
Molecular Formula: C31H46N8O7S
Molecular Weight: 674.81 g/mol
Purity: > 95 % (HPLC)
Form: red oil
Solubility: DMSO, DMF, DCM, MeOH
6-Methyl-Tetrazine-PEG4-Biotin is suitable for the introduction of a biotin moiety to cyclooctene (e.g. TCO)-labeled biomolecules via Cu(I)-free Click Chemistry reaction.
The hydrophilic PEG4 linker reduces or eliminates aggregation and precipitation during the labeling process by increasing the hydrophilicity of the target molecule. It furthermore enhances the accessibility of the biotin moiety and thus the detection efficiency of the biotinylated molecule via fluorescent or HRP-labeled streptavidin or its affinity purification via streptavidin agarose.
Due to the extremely high affinity of biotin towards streptavidin (KD = 10-15M), the biotinylated molecule/streptavidin-interaction is essentially irreversible under physiological conditions.