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Tetrazine-containing Biotinylation Reagents

Tetrazine-containing biotinylation reagents can be used for the labeling of cyclooctene (e.g. TCO) via Cu(I)-free Click Chemistry.

Two Tetrazine versions with different reactivities and stability characteristics are available to meet specific application requirements. Tetrazine reagents are the ideal choice if a rapid reaction kinetic is the key aspect whereas 6-Methyl-Tetrazine reagents are ideally suited if an improved chemical stability is required [1]. Both biotinylation reagents contain a PEG linker for increased molecule solubility and optimal streptavidin binding.

 

Selected References

[1] Karver et al. (2011) Synthesis and Evaluation of a Series of 1,2,4,5-Tetrazines for Bioorthogonal Conjugations. Am. Chem. Soc. 22:2263.