DBCO-PEG 20 kDa

Structural formula of DBCO-PEG 20 kDa

For research use only!

Shipping: shipped at ambient temperature

Storage Conditions: store at -20 °C
store undissolved, for use prepare a fresh solution

Shelf Life: 12 months after date of delivery (undissolved)

Molecular Weight: 20 kDa

Purity: > 95 % (HPLC)

Form: white to slightly grey solid

Solubility: DCM, DMF, DMSO, water

Description:
A reagent for the PEGylation the azide-containing biomolecules via Copper-free click reaction.

Important Product Information

• Avoid buffers that contain azides, which can react with DBCO.
• Reactions of DBCO and azides are more efficient at high concentrations and temperatures (i.e. 4 - 37 °C). Typical reaction times are less than 12 hours, however, incubating for longer can improve efficiency.

Copper-free click reaction:

• Prepare the azide-containing protein in reaction buffer.
• Add DBCO-PEG conjugate to azide-containing sample.
• Recommendation: Add 2 - 3 mol equivalents of DBCO-PEG conjugate to 1 mol equivalent of azide-containing protein.
• Incubate the reaction mixture at room temperature for 4 - 12 hours for smaller DBCO-PEG (5, 10 kDa) and 12 - 24 hours for larger ones (20, 30, and 40 kDa). The incubation at 4 °C requires 6 - 24 hours.
• The reaction mixture is now ready for purification by size exclusion chromatography if required.