Dibenzocyclooctyne (DBCO)^*-containing (desthio) biotinylation reagents can be used for the labeling of Azide-tagged biomolecules via Cu(I)-free strain-promoted Alkyne-Azide Click Chemistry (SPAAC).

A number of (desthio) biotinylation reagents with

• different solubility characteristics (PEG- and Sulfo-linker for increased molecule solubility)
• different cleavability characteristics (chemically or photocleavable linke)

are available to fit specific application & molecule requirements.

^*DBCO is also known as ADIBO (= Azadibenzocyclooctyne) or DIBAC (= Dibenzoazacyclooctyne)

Table 1: Available DBCO-containing (Desthio) Biotinylation Reagents