DBCO Reagents - (Desthio) Biotinylation Reagents

Dibenzocyclooctyne (DBCO)^*-containing (desthio) biotinylation reagents can be used for the labeling of Azide-tagged biomolecules via Cu(I)-free strain-promoted Alkyne-Azide Click Chemistry (SPAAC).

A number of (desthio) biotinylation reagents with

different solubility characteristics (PEG- and Sulfo-linker for increased molecule solubility)

different cleavability characteristics (chemically or photocleavable linke)

are available to fit specific application & molecule requirements.

^*DBCO is also known as ADIBO (= Azadibenzocyclooctyne) or DIBAC (= Dibenzoazacyclooctyne)

Click Chemistry background information

CLICKable (Desthio)Biotinylation Reagents

Table 1: Available DBCO-containing (Desthio) Biotinylation Reagents

Label	DBCO
Biotin	DBCO-PEG₄-Biotin Sulfo-DBCO-Biotin
Biotin with chemically cleavable linker	Diazo Biotin-DBCO Dde Biotin-DBCO
Biotin with photocleavable linker	Photocleavable Biotin-DBCO Photocleavable Biotin-Sulfo-DBCO
Desthiobiotin	DBCO-PEG₄-Desthiobiotin